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Microscopy

What would it look like to go from 10 million light years from Earth to an atom in an oak tree leaf in Tallahassee, Florida? Let's see.shall we? Orders of Magnitude. Vocabulary. Magnification - making an image appear larger than its actual size Resolution - how clear an image isMicroscope - inst

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Microscopy

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    1. Microscopy

    2. What would it look like to go from 10 million light years from Earth to an atom in an oak tree leaf in Tallahassee, Florida? Lets see.shall we? Orders of Magnitude

    3. Vocabulary

    4. Microscope History

    5. How do we see?

    6. Optical (Light) Microscopes Light passes through specimen ~ size of a bacterium Magnification: up to 2,000 x Can be used to study live cells

    8. Electron Microscopes Uses electrons to form an image of an object 100x light microscope i.e. viruses and bacteria Magnification: up to 200,000 x Can only be used on dead cells

    9. 2 Kinds of Electron Microscopes Transmission Electron Microscope (TEM) Electron beam through thin section of specimen Flat image Scanning Electron Microscope (SEM) Specimen - whole Electrons bounce off sample 3D Image

    10. What in the World?

    11. What in the World?

    12. SEM Images

    13. SEM Images

    14. Probe Microscopes Scanning Tunneling Microscope (STM) Uses probe and weak electrical current Measures on an atomic scale 3D Image

    15. The Compound Light Microscope Eyepiece Lower-power objective Nosepiece Body tube Coarse adjustment Fine adjustment Stage Stage clips Diaphragm Light source Arm base

    16. Using a Compound Light Microscope 1) Total magnification = Eye piece x objective lens Example: (10x) x (30x) = 300x Magnification Examples Cover slips - No bubbles! Focusing - Coarse 1st!

    17. Questions How do you place a cover slip on a slide? What objective do you start your viewing with? As you focus, what do you need to be sure to watch out for?

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