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بسم الله الرحمن الرحيم

بسم الله الرحمن الرحيم. Pharmaceutical Biotechnology PHG 424 RECOMBINANT DNA TECHNOLOGY Dr. Magdy Muharram. Basic Structure of Nucleic Acids. 5’. 1’. 4’. Purine. Pyrimidine. 3’. 2’. Ribose, Deoxyribose. Adenine Guanine Thymine Cytosine Uracil. Monophosphate Diphosphate

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بسم الله الرحمن الرحيم

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  1. بسم الله الرحمن الرحيم

  2. Pharmaceutical Biotechnology PHG 424 RECOMBINANT DNA TECHNOLOGY Dr. Magdy Muharram

  3. Basic Structure of Nucleic Acids 5’ 1’ 4’ Purine Pyrimidine 3’ 2’ Ribose, Deoxyribose Adenine Guanine Thymine Cytosine Uracil Monophosphate Diphosphate Triphosphate Nucleoside (Adenosine) Nucleotide (Adenosine monophosphate, AMP)

  4. Nucleotides Linked by Phosphodiester Bond P R P R P R P R P R P R 1 2 3 2- 2- 5’ O 4 PO4 PO4 - O-P=O 5 O 6 3’ OH 5’ 5’ A kind of phospho-polysaccharides 1 3’ OH 2 Phosphodiester bond 3’

  5. The Notation for Nucleic Acids A T C G A T C G 1’ 2’ 3’ 5’ 5’ 3’ P OH 5’pApTpCpGpApTpCpG-OH3’ B 5’pATCGATCG-OH3’ R ATCGATCG P

  6. The Two Chains of DNA Are Antiparallel 5’ 3’ 5’pApTpCpGpApTpCpG-OH3’ 5’pCpGpApTpCpGpApT-OH3’ 3’ 5’

  7. Characteristics of Double Helix 3’ 5’ 1 2 3 4 5 6 7 8 9 10 5’ 3’ 36 Å Large groove Small groove 1 Twist = 10.5 bp

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