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Frank Greenway, M.D. Pennington Center March 3, 2009

Frank Greenway, M.D. Pennington Center March 3, 2009. Angiogenesis. Angiogenesis, growing new blood vessels, is a basic biological process Angiogenesis in adults is used for menses, fetal development and healing wounds.

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Frank Greenway, M.D. Pennington Center March 3, 2009

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  1. Frank Greenway, M.D. Pennington Center March 3, 2009

  2. Angiogenesis • Angiogenesis, growing new blood vessels, is a basic biological process • Angiogenesis in adults is used for menses, fetal development and healing wounds. • Inhibition of angiogenesis in adults should have minimal side effects or toxicity. • Cancer cannot grow past 2mm without angiogenesis and fat needs angiogenesis too.

  3. Human Angiogenesis Assay • Discs are cut from the veins of placentas (after births). These placentas are left over after the delivery of a baby and would be discarded. The discs are put in a clot-like gel. • The discs sprout new blood vessels (initiation) and the new vessels continue to gro over a 2-week period (angiogenic index from 1-16)

  4. Human Tissue Predicts Activity Jung SP et al. Angiogenesis. 4(3):175-86, 2001. Hornick CA et al. Angiogenesis. 6(2):143-9, 2003.

  5. Sweet Leaf Tea • This is a different tea than tea from the plant Camilla, the tea consumed in Britain, the US (black) or in Chinese restaurants (green) • Sweet leaf tea is sweet due to a chemical made by the plant called Ruboside that is a non-caloric sweetener. • Sweet leaf tea is consumed as part of the diet in an area in China and is related to blackberry, a plant called Rubus.

  6. Black Raspberry

  7. Inhibition of Angiogenesis by Sweet Leaf Tea in placental vein assay

  8. Fat is an Angiogenic Tissue • Fat grows and recedes throughout life and is composed basically of fat and vessels. • TNP-470 is a fungus-derived angiogenic inhibitor that makes people very sick. • TNP-470 and other angiogenic inhibitors have been shown to reverse obesity in rodent models of obesity and it does not make the rodents sick.

  9. Intermittent Treatment with TNP-470, 10 mg/kg/d

  10. Human Angiogenesis Assay in Fat Tissue

  11. Active Ingredient .001 M 0.0001 M Control

  12. Sweet Leaf Tea Inhibits Angiogenesis in Human Fat Tissue

  13. p 0.40 RUO-C x 0.35 0.30 q dd v w 0.25 u AU r 0.20 t o s 0.15 n h cc gg y ee ff 0.10 a d z bb c j m hh kk f aa ii i k l jj b e g 0.05 0.00 0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 Minutes Crude Extract with 26 Chemicals

  14. Partially Purified Extract with 13 Chemicals

  15. Purification and Standardization • Purification gets rid of the plant chemicals and other plant components that do not inhibit angiogenesis. Purification resulted in 5 chemicals important for effectiveness • Standardization is the identification of the important chemicals and being sure that every batch of the purified plant has the full amount of the effective chemicals. Standardization is necessary for a consistent product.

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