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Role of the Cytoplasmic Domain in Anabaena Sensory Rhodopsin Photocycling: Vectoriality of Schiff Base Deprotonation. Sineshchekov, O. A.; Spudich, E. N.; Trivedi, V. D.; and Spudich, J.L. Biophysical Journal , 91 , 4519-4527, 2006. Introduction. Rhodopsins
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Role of the Cytoplasmic Domain in Anabaena Sensory Rhodopsin Photocycling: Vectoriality of Schiff Base Deprotonation Sineshchekov, O. A.; Spudich, E. N.; Trivedi, V. D.; and Spudich, J.L. Biophysical Journal, 91, 4519-4527, 2006
Introduction • Rhodopsins • Retinal linked to opsin in Schiff base linkage to lysine residue
Bacteriorhodopsin • With hv, all-trans to cis- retinal • H+ transfer from Lys216 to Asp85 • Glu204 and Glu194 transfer H+ to EC side • Asp 96 reprotonates Lys216 • SRII: