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The measurement of the blood sugar and effects of adrenalin on blood sugar concentration

Exp.7. The measurement of the blood sugar and effects of adrenalin on blood sugar concentration 血糖测定及肾上腺素对血糖浓度的影响. AIM:. 1 、 Study the principle and the methods of the measurement of blood sugar 了解血糖测定的原理和方法；. 2 、 Master the theory of influence of adrenalin on blood sugar concentration

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The measurement of the blood sugar and effects of adrenalin on blood sugar concentration

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  1. Exp.7 The measurement of the blood sugar and effects of adrenalin on blood sugar concentration 血糖测定及肾上腺素对血糖浓度的影响

  2. AIM: 1、 Study the principle and the methods ofthe measurement of blood sugar 了解血糖测定的原理和方法； 2、 Master the theory ofinfluence of adrenalin on blood sugar concentration 掌握肾上腺素对血糖浓度的影响机制

  3. Principle 1. Blood sugar ATP absorption glycogen synthesis blood sugar fat or AA glycogen breakdown other sugar gluconeogenesis glucosuria

  4. Hormone Receptor + + + + + AC AC + cAMP ATP 2、Regulation of blood sugar Pi Phosphorylase b Phosphorylase a 激活 b Kinase -p Pi PKA PKA Glycogen synthase 2C C2R2 Pi -p 失活

  5. Cu COONa CHOH CHOH COOK COONa CHO CHO COOK 3、reaction (1)、 OH- Cu2++ + 2H2O

  6. CHO (CHOH)4 CH2OH COOH (CHOH)4 CH2OH + 2H2O + + Cu2O red 3Cu2O + H3PO4•12MoO3H3PO4•6MoO3•3Mo2O3 + 6CuO Cu blue COONa CHO CHO COOK (2)、 420nm

  7. Operation ? 1、preparation of the blood filtrater without protein ①blooding slowly； ②H2SO4、Na3WO4，dropping and mixing ③Keep the filter paper from wet ！！

  8. 2、 The measurement of blood sugar Reagent (ml) Standard glucose solution filtrater H2O Cu2+ (OH-) solution 磷钼酸试剂 Add H2O to S 2.00 ---- ---- 2.00 2.00 25.0 U1 U2 ---- ---- 2.00 2.00 ---- ---- 2.00 2.00 2.00 2.00 25.0 25.0 O ---- ---- 2.00 2.00 2.00 25.0 Mix up , 100℃ for 8min , cold water for 3min , no shaking Mix up , remain for 2min (release CO2)

  9. Attentions 1、mix up adequately 2、the length of wave λ=420nm

  10. ODU mg% =100× ODS Results

  11. discussion questions 1-4 (P28)

  12. Hormone Receptor + + + + + AC AC + cAMP ATP 2、Regulation of blood sugar Pi Phosphorylase b Phosphorylase a Kinase b-p Pi PKA PKA Glycogen synthase 2C C2R2 Pi -- -p

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