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AML1/Runx1 and oncogenic transformation

AML1/Runx1 and oncogenic transformation. RUNT domain (DNA BINDING). Chr 21. Chr 8. 4 Nervy repressor domains. Translocation (8;21). In human myeloid leukemia =>. RUNT domain. 4 Nervy repressor domains. Lozenge Drosophila. Runt Drosophila. Runx D.rerio. Runx1 G.gallus.

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AML1/Runx1 and oncogenic transformation

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  1. AML1/Runx1 and oncogenic transformation RUNT domain (DNA BINDING) Chr 21 Chr 8 4 Nervy repressor domains Translocation (8;21) In human myeloid leukemia => RUNT domain 4 Nervy repressor domains

  2. Lozenge Drosophila Runt Drosophila Runx D.rerio Runx1 G.gallus RUNX1v2 H.sapiens Runx1 R.norvegicus 0.1 Runx1 M.musculus Mcoffee: multiple sequence alignment and phylogenetic tree RUNX1 alignment

  3. (Drosophila) NO alignment between species for the t(8;21)

  4. Is there a difference between the proteinic folding of AML1 and AML1-ETO??

  5. Predicted structures of AML1-ETO domains NCBI- BLAST RUNT Zf NRH2 domain PDB- models database

  6. Phosphorylation site? Phosphorylation site? AML1-ETO: hydrophobic cluster analysis with drawhca Runt TAFH(NHR1) NHR2 NHR3 zf-NHR4

  7. Scanning for Phosphorylation-sites of AML1: Scansite ERK-1

  8. Conclusions: • Biocomputing study of AML-1 ETO • Sequences comparison vs AML-1 • 3D prediction • Hydrophobic domains prediction • Potential phosphorylation sites of AML1 Comparison with AML1-ETO

  9. .....and now you have to return to the lab and make experiments...........

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