1 / 11

Optical Tweezers

Optical Tweezers. Research performed by: Rachel Patton McCord. at The National Institute of Standards and Technology and Davidson College. Background: Optical Tweezers Physics. Balancing Forces: Scattering Force- “Radiation Pressure” Gradient Force- Restoring force analogy to spring .

elliott
Download Presentation

Optical Tweezers

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Optical Tweezers Research performed by: Rachel Patton McCord at The National Institute of Standards and Technology andDavidson College

  2. Background: Optical Tweezers Physics • Balancing Forces: • Scattering Force- “Radiation Pressure” • Gradient Force- Restoring force analogy to spring Figure from: Svoboda and Block, 1994.

  3. Physics, cont. • Tight focus = largest gradient force • obtained with microscope objective Images from: http://www.olympusmicro.com/primer/java/nuaperture/index.html

  4. Basic Components Laser L1 L2 L3 Objective Lens f1 + f2 f3 16 cm Image created from Physlet at http://webphysics.davidson.edu/Course_Material/Py230L/optics/lenses.htm Physlet by Dr. Wolfgang Christian and Mike Lee

  5. NIST Alignment • Gained experience with alignment by working on a two-laser trap setup.

  6. Results Single Trap Both Traps Together

  7. NIST Optical Tweezers Applications • Liposomes • Stretching/Nanotubes • Microreaction Vesicles • Single Mitochondria Isolation and Sorting • Available for PCR and genetic analysis • Antigen/Antibody Binding • Quantum Dots in Polymersomes • Fluorescence Resonance Energy Transfer and single molecule imaging

  8. Davidson Setup

  9. Results • Trapping microspheres in 3D • 10 mW minimum power from laser (about 2 mW at the slide) • Trapping single celled chlamydomonas Image from: http://www.ucmp.berkeley.edu/greenalgae/chlorophyceae.html

  10. Future Plans • Force calibration • Measuring the swimming force of chlamydomonas • Flagellar mutants Further in the future: • Physics Applications • Stretching- elasticity, determining shear moduli • Biology Applications • More chlamy- phototaxis • Virus adhesion

  11. Acknowledgments • Dr. Kris Helmerson, Jeff Wells, Lara Crigger, Rani Kishore, Joe Reiner at NIST • Dr. John Yukich and Dr. Karen Bernd

More Related