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Microscopic Measurement

Microscopic Measurement. Q: How do you measure the size of a microscopic specimen? A: You will get a close estimation using the following technique…. Step 1 – Measure diameter of FOV. Use a clear ruler with a mm scale to measure the diameter of your field of vision ( FOV ) at low power.  

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Microscopic Measurement

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  1. Microscopic Measurement Q: How do you measure the size of a microscopic specimen? A: You will get a close estimation using the following technique…

  2. Step 1 – Measure diameter of FOV • Use a clear ruler with a mm scale to measure the diameter of your field of vision (FOV) at low power.   • The FOV is estimated to be 5.0 mm in diameter in the picture shown. • No specimen yet! • Convert to micrometers… • 1.0 mm = 1,000 micrometers • The FOV is 5,000 micrometers!

  3. Step 2 – Estimate specimen’s fraction of the FOV • Remove the ruler. • Bring your specimen into focus. • Estimate the fraction of the FOV the specimen covers. • Is it ½? Is it ⅓? Is it ¾? Use your best judgment. 1/3

  4. Step 3 - Restate fraction as a decimal • Simply divide numerator by the denominator • ⅓ = 1 divided by 3 = .3 (repeating)

  5. Step 4 – Multiply decimal by FOV! • The final step is to multiply the decimal you calculated by the diameter across the FOV you measured. • .3 X 5,000 micrometers = 1,500 micrometers • Your answer should always be reported in micrometers!

  6. Sample Problem • FOV = 3.5 mm… = 3,500 micrometers • Specimen = ½ FOV… = 0.5 (as a decimal) • 0.5 X 3,500 micrometers = 1,750 micrometers

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