Protein amount ( μg per fraction)

1. A Imidazole (0-1 M) Protein amount (μg per fraction) Fraction numbers B Protein amount (mg per fraction) NaCl (0-1 M) Fraction numbers Supplemental Fig. 1 Typical chromatography patterns. a HiTrap Chelating HP. An imidazole gradient (0-1 M) was produced. b DEAE-Toyopearl. A NaCl gradient (0-1 M) was performed. Black and red lines show the total protein amounts and the RsDHN amounts, respectively. To make the chromatographic charts, the protein amount in each fraction was determined by measuring the intensities of the protein bands separated by the SDS-PAGE gel stained with Coomassie brilliant blue. Band intensities were analyzed using NIH image software (http://rsb.info.nih.gov/nih-image/).Green bars indicate the fractions collected. Title: Efficient purification of cryoprotectivedehydrin protein from the radish (Raphanussativus) taproot Authors: Hara M, Uchida S, Murata T, Wätzig H Journal: Eur Food Res Technol

2. AA_RS1A356JQ 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGERKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS1AW77TF 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS1CA51JQ 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEERKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS1FS64TF 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDYKH 70 AA_RS1FZ84JQ 1:MAGIINKIGDALHIGVGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS1FZ84TF 1:MAGIINKIGDALHIGVGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS2F763JQ 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS2F763TF 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS2FS53JQ 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS2FS53TF 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS2FV08TF 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDLKH 70 AA_RS3FZ43JQ 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 AA_RS3FZ43TF 1:MAGIINKIGDALHIGGGHKEDEHKKEEHKKEEHKKHADEHKSGEHKEGIVDKIKDKIHGGEGHSSGDHKH 70 ***************.****************.***********.**********************.** AA_RS1A356JQ71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS1AW77TF71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS1CA51JQ71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS1FS64TF71:DGEKKKKKDKKEKKHHDDGRHSSSSDSDSD 100 AA_RS1FZ84JQ71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS1FZ84TF71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS2F763JQ71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS2F763TF71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS2FS53JQ71:DGEKKKKKDKKEKKHHDDGHHSGSSDSDSD 100 AA_RS2FS53TF71:DGEKKKKKDKKEKKHHDDGHHSGSSDSDSD 100 AA_RS2FV08TF71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS3FZ43JQ71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 AA_RS3FZ43TF71:DGEKKKKKDKKEKKHHDDGHHSSSSDSDSD 100 *******************.**.******* Supplemental Fig. 2 Thirteen full-length cDNA sequences from a radish, shown as orthologs of ArabidopsisdehydrinAtHIRD11 (At1g54410) on the Arabidopsis information resource web site (TAIR, http://www.arabidopsis.org/). Alignment was done using GENETYX software (Genetyx, Tokyo, Japan). Letters starting from AA_RS represent the names of the corresponding cDNAs. Asterisks represent amino acids that are identical among the 13 sequences. Sequences of the 5 clones shown in red are totally identical. Title: Efficient purification of cryoprotectivedehydrin protein from the radish (Raphanussativus) taproot Authors: Hara M, Uchida S, Murata T, Wätzig H Journal: Eur Food Res Technol