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Jordi Pelkmans Amanda Maurits

Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N -linked Fc oligosaccharides: the high-mannose, hybrid, and complex types. Jordi Pelkmans Amanda Maurits. Antigen-IgG1-Fc γ RIIIa binding Antibody Dependent Cellular Cytotoxicity (ADCC).

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Jordi Pelkmans Amanda Maurits

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  1. Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types Jordi Pelkmans Amanda Maurits

  2. Antigen-IgG1-FcγRIIIa bindingAntibody Dependent Cellular Cytotoxicity (ADCC) FcγRIIIa

  3. Antigen-IgG1-C1q bindingComplement Dependent Cytotoxicity (CDC) C1q

  4. Human IgG1

  5. Three types of N-linked oligosaccharides • High-mannose • Hybrid • Complex All types can be fucosylated

  6. Different types of oligosaccharides created in this article No difference in binding of the variable part of the IgG1 was observed

  7. Difference in binding affinity to FcγRIIIa

  8. Difference in binding affinity to FcγRIIIa

  9. Difference in ADCC activity

  10. Contamination of different types of oligosaccharides influences activity of Fu(-) Complex type

  11. Difference in CDC activity

  12. C1q binds best to Complex type

  13. Plasma clearance of different types

  14. The FcRn receptor • Binds IgG1 and prevents its degradation

  15. Conclusion

  16. Questions?

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