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8. Team Cherry Picker Blake Hondl Amit Mehta Jon Millin Ryan Pope

9. Team Small Molecule Blake Hondl Amit Mehta Jon Millin Ryan Pope

10. Client & Advisor Noël R. Peters, M.S. Keck-UWCCC Small Molecule Screening Facility Professor Willis TompkinsDepartment of Biomedical Engineering

11. Abstract • High throughput screening (HTS) involves secondary testing of chemicals that initially show desired characteristics • Done manually and prone to errors • Goal is to design a device to guide a micropipette user to a single microtiter well • The device will increase efficiency and reduce user error • Device implementations • Java Application • LCD Screen • User Interface

12. Problem Statement • Design a device to guide a micropipette user in the transfer of small volumes of compounds between 384-well microtiter plates • The device will increase the efficiency of transfers and reduce user error

13. Background • High Throughput Screening • Used in cancer and RNA research • Secondary testing performed on the chemicals that initially show promise • Use of plate reader to determine potential “hits” EnVison® Plate Reader

14. Motivation • Secondary testing is typically done manually, making it difficult and prone to errors • Row and column identification is necessary • Interior wells are difficult to locate • Errors lead to increased time costs • Only step in the process that is prone to significant human error

15. Matrix Memowell® 96 well pipetting aid Lights up 4 wells when used with 384-well plate Costs approx. $1000 Quadra Cherry Picker Automated Costs $150,000 Current Competition http://www.matrixtechcorp.com http://www.tomtec.com

16. Design Constraints • Must interface with MicrosoftExcel and user • Effectively guide the user to the appropriate wells • Minimize required bench space • Dimensions smaller than 8.5” x 11” • Withstand exposure to various chemicals and reagents • Dimethyl Sulfoxide • Lightweight (<10 lbs) and inexpensive (<$1000)

17. Final Design • Java Application • User input to locate wells in one or two plates • Excel input from spreadsheet (future work) • LCD Screen to show output from Java Application • Lines (cross-hairs) displayed to identify wells to user

18. Final Design • Protection of the LCD screen • Hard acrylic sheet protects LCD screen from pressure and possesses good optical clarity • Polypropylene sheet to protect from the Dimethyl Sulfoxide buffer • Maintaining the position of well plates on LCD screen • Two sets of tracks guide the movement of “arms” which will fix the plates in place

19. Prototype Construction • Purchased refurbished LCD screen • 10.4” Screen • Inverter • Ribbon cable • Needs to be refreshed at 60 Hz • Too fast for serial or USB port

20. Prototype Construction • Acquired materials • Laptop: Samsung SENS 800 • Aluminum sheet metal • Polypropylene cover • Non-glare 1/8” acrylic sheet • Detached screen from base • Purchased components to extend screen • 30 connector HD ribbon cable

21. User interface User may choose between: User input or MicrosoftExcelfile input Using one well plate or two Full screen display Displays rectangle for definition of well plate boundaries Displays intersecting lines according to user input, illuminating a single well Programming Well plate on LCD screen

22. Advantages Ease of use Manufacturing Adaptability Cost Marketability Reduces errors Increases productivity Disadvantages Requires computer interface Requires purchasing of hardware as well as software. Software upgrades to enable adaptability Design Analysis

23. Cost Analysis • Used Laptop from SWAP =$250.00 • 1/16” Aluminum Sheet (8 ft2) =$33.36 • Acrylic Sheet (2) =$ 3.90 • Polypropylene cover =$ 0.20 • Flat Ribbon Cable & Connector =$16.71 • Miscellaneous Components =$ 7.00 Total=$311.17 • Cost to purchase a new LCD flat screen for a computer $300 (Future Work)

24. Future Work • Implement additional programming code • Allow the program to take input from an Excelfile • Conduct initial testing on laptop prototype • Create interface between prototype and PC • Design an optimal structure for the prototype, emphasizing ease of use for the user • Confirm prototype’s resistance to chemical solvents encountered in the lab

25. References • “Digikey.” Digi-key Corporation. http://www.digikey.com. Retrieved on April 15, 2004. • “Java Excel API Tutorial”. http://www.andykhan.com/jexcelapi/tutorial.html. • “LCD Basics: Monitor Technology 101.” ViewSonic. http://www.viewsonic.com/monitoruniversity/lcdbasics.htm. • “LCD Video Controller.” Subassembly Product Guide. http://www.trans2000.com/manual/adboard_manual.pdf. • “Matrix Memowell.” Matrix Technologies. http://www.matrixtechcorp.com. Retrieved on January 23, 2004. • Peters, Noël. Personal Interview. January 30, 2004. • “Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept. LG. Philips LCD Co., Ltd. http://www.jacoflatpanels.com/lcdpdfs/LB104V03-A1_CAS(Ver0.1).pdf. • “Quadra Cherry Picker.” Tomtec. http://www.tomtec.com. Retrieved on January 29, 2004. • “Samsung.” Samsung Electronics. http://www.samsungusa.com. Retrieved on April 22, 2004. • Schowalter, Jeff. Personal Interview. March 26, 2004. • “Small Molecule Screening Facility.” UW-CCC. http://www.medsch.wisc.edu/smsf/Index.htm. Retrieved on January 23, 2004. • Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third Edition)” and imported classes from JavaBook.