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A combination of biomarkers to identify senescent cells

A combination of biomarkers to identify senescent cells. Senescence associated Beta Galactosidase activity (SA-bgal) is increased during replicative aging of cells and other conditions. Nucleoli number can accurately distinguish between young and old cells

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A combination of biomarkers to identify senescent cells

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  1. A combination of biomarkers to identify senescent cells • Senescence associated Beta Galactosidase activity (SA-bgal) is increased during replicative aging of cells and other conditions. • Nucleoli number can accurately distinguish between young and old cells • Using a combination of these markers, we can identify senescent cells in culture and (hopefully) tissue.

  2. Relationship of SA β-gal / Safranin O and Various Conditions Young cells Quiescent cells “Stressed” cells Senescent cells β-gal+ β-gal- Quiescent cells “Stressed” cells Senescent cells Young cells Safranin-O ≥3 nucleoli Safranin-O ≥3 nucleoli 1-2 nucleoli Young cells Quiescent cells “Stressed” cells Senescent cells

  3. Relationship of SA β-gal / Acridine Orange and Various Conditions Young cells Quiescent cells “Stressed” cells Senescent cells

  4. Safranin – O nucleolar staining Quiescent cells “Stressed” cells Senescent cells

  5. Safranin – O nucleolar stainingMBMSC Early Wrn/Terc -/-

  6. In Vivo AgNOR staining

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