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Ways of Studying Cells A. Visualization of Cells

Ways of Studying Cells A. Visualization of Cells. BSCI 420 Advanced Cell Biology Sept 11, 2002 “Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln (In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath).

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Ways of Studying Cells A. Visualization of Cells

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  1. Ways of Studying Cells A. Visualization of Cells BSCI 420 Advanced Cell Biology Sept 11, 2002 “Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln (In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath)

  2. Resolving Power

  3. Bright-field light microscope

  4. Resolution depends on the Numerical Aperture

  5. Types of LM: A) Bright field B) Phase contrast

  6. Types of LM: C) Diff. Interference Contrast D) Dark field

  7. Bright field microscopy of stained section of kidney tubules

  8. Bright field vs. Phase contrast

  9. Constructive and destructive interference of light

  10. Fluorescence Microscope

  11. Fluorescence image of a cell in mitosis labeled w 3 tags: Green: fluorescein-labeled anti-tubulin (MTS) Red: anti- centromere protein Blue: DAPI (DNA)

  12. Indirect Immunocytochemistry

  13. Excitation and Emission wave-lengths Note emission w-l is always > than excitation w-l. So they have lost energy (Energy = 1/wave-length

  14. Immunofluorescence of individual MTs

  15. Images of Conventional (A) vs Confocal (B) Fluorescence (Drosophila embryo stained w fluorescent probe against actin)

  16. GFT tagged talin in trichrome cells of Arabidopsis

  17. Dynamics of GFP-labeled Cajal bodies in the nucleus

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