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Biological Safety Cabinets

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Biological Safety Cabinets

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    1. Biological Safety Cabinets Marcy Huey August 2009

    3. Hazard Levels? What are you talking about??!! Hazard Group 1 2 3 4 (of pathogens) Containment Level 1 2 3 4 (of laboratories) Class 1 2 3 4 (of Genetic Modification) CLASSI II III (of Safety Cabinets)

    4. The terminology –‘Safety’Cabinets This is one of the most common mistakes made Tissue Culture Hood, Laminar Flow, Glove Box, Fume Hood, Hoods, Cabinets, Isolator – not all exactly the same Microbiological Safety Cabinet Class I, II or III Also called Horizontal Laminar Flow/ Vertical Laminar Flow Sometimes called Isolators Or Fume Cupboards

    5. ‘Containment Level’of Laboratories CL1:-“I’ll just do it on the bench over there…..” CL2:-“OK, so I’ll have to use the hood…….” CL3:-“That’s that room along the corridor that no-one goes in?” CL4:-“Spacesuits?”

    6. ‘Containment Level’of Laboratories CL1:-easy to clean, impervious resistant surfaces, disinfectants to hand,minimise aerosols, door shut during work. GLP -lab coats and PPE, no mouth pipetting, eating etc etc, hand wash sink, safe disposal routes& in addition to the above…. CL2:-authorised access only, specified disinfection procedures, safe storage of biological agents, work producing aerosols must be in MSC, access to incinerator for some bio waste, 24m3 space per person, autoclave, negative airflow during work if possible, usually require BSC

    7. ‘Containment Level’of Laboratories CL3:-Must be maintained at negative pressure, sealable for fumigation,& extracted air must be HEPA filtered.Must be separated from any other activities in the building.Secure access & list of authorised personnel posted on entrance. Observation window.Laboratory to contain its own equipment, BSC & autoclave.Impervious floor as well as benches.Written contingency plans in case of spillage. Written training records.& in addition to the above….UA doesn’t currently have any CL3 labs. CL4:-The University has no CL4 labs.

    8. BSC vs Fume Hood Biological Safety Cabinets (BSC) provide: Protection for the user and the product HEPA filtration of air into cabinet and exhaust air Fume hoods provide: Removal of contaminated air Protection for the user from chemicals

    9. BSCs vs Fume Hoods Biological Safety Cabinets provide user and product protection via HEPA filtered air circulation. Fume Hoods provide user protection via removal of contaminated air to external exhaust.

    10. Biological Safety Cabinets Must be properly maintained and regularly serviced Must be certified annually and when new, moved, or repaired Must be used in conjunction with good microbiological techniques Provide protection for personnel, product, and environment

    11. General Cabinet Information Types of Biological Safety Cabinets Choose the correct Biological Safety Cabinet for your experiments. Contact the Department of Environmental Health and Safety before purchasing a new cabinet or beginning a new project for help in selecting the appropriate cabinet. Biosafety in Microbiological and Biomedical Laboratories http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm

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