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G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4

DEVELOPMENT OF A NEW APPARATUS FOR THE ASSESSMENT OF HEALTH IMPACT FROM EXPOSURE TO FIRE COMBUSTION PRODUCTS. G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4 1 School of Civil and Environmental Engineering 2 School of Chemical sciences

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G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4

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  1. DEVELOPMENT OF A NEW APPARATUS FOR THE ASSESSMENT OF HEALTH IMPACT FROM EXPOSURE TO FIRE COMBUSTION PRODUCTS G. Chattopadhyay1, Paul Hallahan2, Paul Brockbank3, F. Lestari4, A. Hayes4, A. Green4 1School of Civil and Environmental Engineering 2School of Chemical sciences 3School of Chemical Engineering and Industrial Chemistry 4School of Safety Science The University of New South Wales, Sydney 2052, Australia

  2. Fire combustion dynamics measurement (TGA analysis) In vitro cytotoxicity measurement Impinger Direct exposure Sampling for chemical analysis GC-MS Chemical analysis Thermal desorption Cryo-focusing TECHNIQUES INCORPORATED IN THE APPARATUS

  3. Basic components of the apparatus TGA data Acc Flow control Mixing chamber Tube furnace Load cell for TGA data Frame

  4. Tube Furnace Quartz tube 1000 mm long X 40 mm diameter

  5. Load Cell for TGA data TGA data cable Gas inlet Bottom of the quartz Tube furnace Ground glass joint Fits onto the tube furnace Hole in polycarbonate Enclosure to tare balance Polycarbonate enclosure

  6. Mixing Chamber Exhaust line from mixing chamber to lab suction system Sampling lines Mixing Chamber : 310 (L) X 300(W) X 310 (H) Volume: 28.83 (L) Exhaust line to lab suction system from Tube furnace enclosure

  7. Flow control components Flow control for gas and sampling streams Temperature control for Direct exposure cells Impinger Suction pump for Collecting samples Temperature control For tube furnace

  8. Experiment flowchart Air sampling using culture medium (serum free) 96 well plates MTS assay Impinger method

  9. outlet inlet membrane Direct exposure at the air/liquid interface Snapwell insert Membrane preparation Navicyte chamber MTS Assay

  10. Chemical analysis for combustion products ATD-GCMS Sorption in a solid sorbent

  11. To GC column transfer line Cold trap at -20° C Tube Desorb

  12. To GC column transfer line Trap Desorb

  13. Preliminary result CO2 Direct exposure Impinger method

  14. PMMA cytotoxicity under non-flaming condition at different post exposure time

  15. Potential applications • Fire toxicity testing for materials or products • Screening and early sign of fire hazard to fire fighters (biosensor) • Toxicity studies for gases, particulates, non-hydrosoluble compounds, complex mixtures, and organic volatile compounds • Many applications in odour analysis and odour management

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