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Lugol’s Iodine

Lugol’s Iodine. Place a sample of the food in distilled water in a test tube. Add 3 drops of Lugol’s iodine in each test tube. Agitate sample and mix thoroughly. Observe color change If starch is present, blue-black precipitate will form. Biuret Solution. Add 40 drops of the test liquid

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Lugol’s Iodine

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  1. Lugol’s Iodine • Place a sample of the food in distilled water in a test tube. • Add 3 drops of Lugol’s iodine in each test tube. Agitate sample and mix thoroughly. • Observe color change • If starch is present, blue-black precipitate will form

  2. Biuret Solution • Add 40 drops of the test liquid • Add 3 drops of Biuret reagent solutions to each test tube. • Shake gently to mix • Note color change

  3. Sudan III stain • In test tube add equal parts pf test liquid and water to the tube is half filled • Add 3 drops of Sudan III stain to each test tube and shake gently to mix • A red-stained oil layer will separate out and float on the water surface if fat is present.

  4. Benedict’s Solution • Add 40 drops of liquid to be tested • Add 10 drops of Benedict’s solution into each test tube • Carefully heat the test tubes by suspending in a hot water bath for 5 minutes • Note any color change

  5. Benedict’s Solution

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