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Candidate selection

B. A. Candidate selection. FLcDNA finishing. 90,368 high-quality 3’-end ESTs. 5,926 FL candidates. Filter for cloning structure missing 5’ SSPA (stringent) missing polyA tail (stringent). CAP3 assembly. 35,011 putative unique transcripts (PUTs). 5,653 FL candidates.

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Candidate selection

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  1. B A Candidate selection FLcDNA finishing 90,368 high-quality 3’-end ESTs 5,926 FL candidates • Filter for cloning structure • missing 5’ SSPA (stringent) • missing polyA tail (stringent) CAP3 assembly 35,011 putative unique transcripts (PUTs) 5,653 FL candidates • Filter for rearray accuracy • mixed well • rearray to correct plate PUTs lacking clones derived from FL libraries excluded 7,346 FL candidates 5,532 FL candidates • Filter for FL candidate criteria • short 5’- and/or 3’-end ESTs (Q20 < 100 nt) • shorter at 5’ end than public poplar ESTs • missing 5’ SSPA • missing polyA tail • Filter for sequencing problems • hard stop 5,525 FL candidates • Filter for clone features • sequence vs. gel data • chimeric 5,926 FL candidates 5,443 FL candidates + 4,664 (79%) finished 779 (13%) incomplete 483 (8%) aborted

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