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Laboratory Safety

Laboratory Safety. Safety. If there was an accident in your science lab, would you know what to do? . Do you Know?. What should you do if your lab partner had a chemical on his or her fingers and accidentally rubbed their eyes?  What is the proper procedure for handling broken glassware?.

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Laboratory Safety

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  1. Laboratory Safety

  2. Safety • If there was an accident in your science lab, would you know what to do? 

  3. Do you Know? • What should you do if your lab partner had a chemical on his or her fingers and accidentally rubbed their eyes?  • What is the proper procedure for handling broken glassware?

  4. Be Aware of what’s going on!!!!! • The purpose of this is to make you aware of the safety procedures that must be followed in the laboratory setting. • Learn to avoid dangerous situations in the lab. 

  5. What we will learn? • Become familiar with Lab Safety Rules and Symbol • Become familiar with reading an MSDS • Create a Laboratory Safety Project • Create a Laboratory Safety TEST

  6. Let’s look at your lab safety handout. • This handout is taken from NSCC. Thanks Dr. Dolan!!

  7. Material Safety Data Sheet • What is it? What does it tell me? • Go to your worksheet. • Where to Find MSDS's on the Internet   http://www.ilpi.com/msds/index.html

  8. It’s your RIGHT to KNOW • Any chemical or biological substance we deal with you have the RIGHT to Know if it can harm you and how to prevent harm.

  9. OSHA • Occupational Safety Health Association mandated that all employee’s have the RIGHT to KNOW for their well being and safety about any materials that you handle.

  10. Universal or Standard Precautions Every specimen should be treated as though it is infectious

  11. Personal Protective Equipment • Gloves • Gowns • Goggles • Masks • booties

  12. Blood borne Pathogen • Any disease causing organism found in blood or body fluids transmitted by blood or body fluids.

  13. HBV • Hepatitis B virus • Hepa-liver -it is means inflammation • Disease that causes inflammation of the liver and could lead to death • HBV vaccine (3 series Vaccine)

  14. HIV • Human Immunodeficiency virus • This virus can lead to AIDS • Acquired Immune Deficiency Syndrome

  15. HIV • Fever • Chills • Cough • Night sweats

  16. AIDS will develop when.. • Opportunistic infection occur. • They are infections caused due to lack of T-cells in the immune system. Your body can not fight them off.

  17. So as workers in a Laboratory • You must be aware of Blood borne pathogens.

  18. Why Is Safety Important? • Coming in contact with human blood or blood products is potentially hazardous. • Safety involves taking precautions to protect you and the client against infection.

  19. Other people who may come in contact with testing by-products Protect integrity of test products Protect environment from hazardous material What Else Needs Protection? 2.1

  20. Apply Safety Practices Throughout the Testing Process • Before Testing (Pre-analytical) • Specimen collection • Specimen preparation • Specimen transport • Testing (Analytical) • Testing • After Testing (Post-analytical) • Disposal

  21. Develop Personal Safe Work Habits • Wash hands before and after testing each patient • Wear a fresh pair of gloves with each patient • Wear lab coat or apron • Dispose of contaminated sharps and waste immediately after testing

  22. Safe habits • Read cautions signs. • Biohazard • Flammable • Toxic • Carcinogen • Many more

  23. Develop Personal Safe Work Habits (Cont’d) • Pipetting by mouth is strictly forbidden • Never eat, drink or smoke at the test site • Keep food out of the laboratory/testing site refrigerator

  24. Maintain Clean & Orderly Work Space • Keep work areas uncluttered and clean • Disinfect work surfaces daily • Restrict or limit access when working • Keep supplies locked in a safe and secure area • Keep emergency eye wash units in working order and within expiry date Biohazard

  25. Take Precautions to Avoid Needle Stick Injury What can cause needle stick injury? • Lack of concentration • Inexperience • Lack of concern for others • Improper disposal of sharps

  26. Drop Used Sharps in Special Containers WASTE

  27. Do’s and Don’ts: Sharps and Waste Containers • Do Not break, bend, re-sheath or reuse lancets, syringes or needles • Do Not shake sharps containers to create space 2.4

  28. Do’s and Don’ts: Sharps and Waste Containers What’s wrong with this picture?

  29. never Place Needles or Sharps in Office Waste Containers 2.4

  30. Sharps Containers Must Be: Placed near workspace Closed when not in use Sealed when ¾ full

  31. Disinfect Work Areas with Bleach Disinfection • Kills germs and pathogens • Keeps work surface clean • Prevents cross-contamination • Reduces risks of infection

  32. General lab use - Hypochlorite Solutions Different Cleaning Jobs Require Different Bleach Solutions* You should have 10% bleach readily available at your test site. * WHO Laboratory Biosafety Manual

  33. In Case of a Spill or Splash • Wear clean disposable gloves • Immediately and thoroughly wash any skin splashed with blood • Large spills- Cover with paper towels and soak with 10% household bleach and allow to stand for at least 5 minutes • Small spill - Wipe with paper towel soaked in 10% bleach • Discard contaminated towels in infectious waste containers

  34. In Case of an Accident • What types of accidents can happen? • Potential Injury, i.e., needle sticks, falls • Environmental, i.e., splashes or spills • Equipment damage • What should you do? • Report to supervisor immediately • Assess & take action • Record using form • Monitor situation

  35. What is safety? Why is it important? What does bio-hazard mean? What is the #1 standard precaution you must take when dealing with specimens? What are some examples of safety practices related to personal habits? Work space? What are the rules related to handling sharps and waste? How do you prepare a 10% bleach solution? What do you do if there is a spill? What do you do when an accident occurs? Summary

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