1.03k likes | 3.57k Views
Preformation vs. Epigenesis. Similarities Among Vertebrate Embryos. Development by Kingdom. Sexual Reproduction in the Unicellular Protist Chlamydomonas. Development in the Multicellular Protist Volvox. Life Cycle of the cellular slime mold, Dictyostelium discoideum.
E N D
Sexual Reproduction in the Unicellular Protist Chlamydomonas
Life Cycle of the cellular slime mold, Dictyostelium discoideum
adult Urochordates- Tunicates (sea squirts) Ciona intestinalis Styela partita larva
Developmental Toolbox • Observational Tools • Fate mapping, lineage analysis • Experimental Tools • Physical manipulation • Ablation, isolation, transplantation • Genetic manipulation • Mutations, gene interference, mosaics, chimerics
Developmental Toolbox • Observational Tools • Fate mapping, lineage analysis • Experimental Tools • Physical manipulation • Ablation, isolation, transplantation • Genetic manipulation • Mutations, gene interference, mosaics, chimerics
Embryo Fate Mapping Techniques • Direct visualization of transparent embryos • Vital dye staining • Injection of fluorescent dye or radiolabel • Genetic marking
Experimental Manipulations Physical: Ablation Isolation Transplantation Genetic
Ablation Experiment (aka. Defect or Removal)
Isolation Experiment (aka. Ligation)