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PCR – The Polymerase Chain Reaction Theory and Applications

PCR – The Polymerase Chain Reaction Theory and Applications. A three-day short course…. Dave Palmer, Bio-Rad dpalmer@zdap.com http://zdap.com/pcr. April 4, 2009. AM. Lec: PCR History Lec: GMO Investigator Kit Background Lab: Preparation of GMO food samples Lec: How PCR Works

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PCR – The Polymerase Chain Reaction Theory and Applications

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  1. PCR – The Polymerase Chain ReactionTheory and Applications A three-day short course… Dave Palmer, Bio-Rad dpalmer@zdap.com http://zdap.com/pcr

  2. April 4, 2009 AM • Lec: PCR History • Lec: GMO Investigator Kit Background • Lab: Preparation of GMO food samples • Lec: How PCR Works • Lab: GMO PCR Reaction Preparation PM

  3. April 18, 2009 AM • Lec: Recap • Lec: Electrophoresis Review • Lab: Set up GMO PCR agarose gels • Lec: PCR Primer Design • Lab: GMO PCR results analysis • Lec: Crime Scene PCR kit overview • Lab: Set up Crime Scene PCR reactions • Lec: Advanced PCR and Troubleshooting PM

  4. April 25, 2009 AM • Lec: Recap • Lab: Set up CSI agarose gels • Lec: Real-Time PCR • Lab: Analyze and interpret gels • Lab: Set up real-time PCR reactions • Final questions? • Final Exam PM

  5. Course Goals • Introduction to PCR • Basic PCR methods • PCR troubleshooting • Agarose gel electrophoresis • Understanding of real-time PCR • Basic PCR lab skills • Pipetting technique • Basic DNA extraction • Agarose gel electrophoresis • Experimental design and use of controls

  6. Course Evaluation • 25% Test One (April 4) • Take-home multiple choice, 25 questions. • 25% Test Two (April 18) • Take-home multiple choice, 25 questions. • 50% Final Exam (April 25) • Open-book test, short answer, ~30min

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