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IN-VITRO Skin sensitization test

IN-VITRO Skin sensitization test. Haptenation. Resorption. Sensitization index. Irritation index. Keratinocytes NCTC2544. 2. 3. 4. 3. 2. 1. 1. Fixing. Detection. Cytokine production. Activation / Migration. Presentation. Xenobiotic. Resorption. Endogenous protein.

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IN-VITRO Skin sensitization test

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  1. IN-VITRO Skin sensitization test

  2. Haptenation Resorption Sensitization index Irritation index Keratinocytes NCTC2544

  3. 2 3 4 3 2 1 1 Fixing Detection Cytokine production Activation / Migration Presentation Xenobiotic Resorption Endogenous protein Keratinocyte Haptenation Dendritic cell (Langerhans cell) Sensitization index T Lymphocyte Irritation index Interleukin 18

  4. 2 3 4 3 2 4 1 1 Fixing Detection Inflammation/ Cell damage Cytokine production Activation / Migration Presentation Xenobiotic Resorption Endogenous protein Keratinocyte Haptenation Dendritic cell (Langerhans cell) Sensitization index T Lymphocyte Irritation index Interleukin 18

  5. 2 3 4 3 2 4 1 1 Fixing Detection Inflammation/ Cell damage Cytokine production Activation / Migration Presentation Xenobiotic Resorption Endogenous protein Keratinocyte Haptenation Dendritic cell (Langerhans cell) Sensitization index T Lymphocyte Irritation index Interleukin 18

  6. Haptenation Resorption Sensitization index Irritation index Keratinocytes NCTC2544

  7. Resorption: During flux Test molecule

  8. Resorption: During flux The resorption kinetics was determined by measuring the appearance of the molecules in the donor compartment

  9. Haptenation Resorption Sensitization index Irritation index Keratinocytes NCTC2544

  10. Haptenation: Addition of urea powder Incubated 1H at 20°C Incubated 4H at 20°C Centrifuge for 2H at 10.000g Solution of the test molecule was mixed with Human serum albumin Centrifuge for 2H at 10.000g Filtrate was quantified Test molecule Endogenous protein

  11. Haptenation Resorption Irritation index

  12. Sensitization: Rinsing Antibodies are deposited in well

  13. Sensitization: Antigens are added

  14. Sensitization: An enzyme-linked antibodies that can react with the antigen is added

  15. Sensitization: The colorless substrate for the enzyme is then added

  16. Sensitization: This coloration was measured by spectrophotometer Development of color indicates the presence of the antigen

  17. Haptenation Resorption Sensitization index Irritation index Keratinocytes NCTC2544

  18. Irritation: Culture cells in micro plate Add to the cell culture the xenobiotic Remove medium and add MTT solution Read with micro plate Reader after incubation Remove MTT and add isopropanol Incubate 3H

  19. Haptenation Resorption Sensitization index Irritation index Keratinocytes NCTC2544

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