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Structure of HRST. Genome length 2903 nt, without transgene Capacity ~6,800 bp. D U3 LTR. LTR. Y. MCS. Plasmid. RRE. P. CMV. SD. Y. MCS. RNA Genome. m7 G-. -A n. RRE. P. CMV. SD. D U3 LTR. D U3 LTR. Y. MCS. Provirus. RRE. P. CMV. SD. MCS. m7 G-. Message. -A n.
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Structure of HRST Genome length 2903 nt, without transgeneCapacity ~6,800 bp DU3 LTR LTR Y MCS Plasmid RRE P CMV SD Y MCS RNA Genome m7G- -An RRE P CMV SD DU3 LTR DU3 LTR Y MCS Provirus RRE P CMV SD MCS m7G- Message -An RRE- rev responsive elementP- internal polypurine tractMCS- multiple cloning sitem7G- 7-methyl guanosine cap SD- splice donor LTR- long terminal repeats An- poly-A tail Y- packaging signal