A N I M A L I T R A N S G E N I C I

1. A N I M A L I T R A N S G E N I C I animali in cui sia stata indotta una deliberata modificazione del genoma

2. ANIMALI TRANSGENICI: campi di applicazione • Studi biologici di base • Ricerca medica • Tossicologia • Biotecnologie • Agricoltura e acquacoltura

3. STRUTTURA DI UN COSTRUTTO TRANSGENICO Sito di splicing Poli-A Promotore cDNA

4. Mediante iniezione pronucleare del costrutto PRODUZIONE DI TOPI TRANSGENICI Mediante ricombinazione omologa in cellule staminali embrionali

7. Costrutto transgenico “tradizionale”: • può contenere fino a 5-50 kbp • si integra casualmente in un singolo sito, sotto forma di 1-50 copie (tandem repeats) • importanza del sito di integrazione • variabilità di espressione nelle diverse linee founder

8. Yeast Artificial Chromosomes (YACs): • possono contenere fino a diverse centinaia di kbp • espressione più uniforme e prevedibile • relativa importanza del sito di integrazione • scarsa stabilità durante la manipolazione

9. Bacterial Artificial Chromosomes (BACs): • possono contenere fino a 100-150 kbp • espressione più uniforme e prevedibile • relativa importanza del sito di integrazione • stabilità durante la manipolazione

10. Mouse Mammary Tumor Virus ONCOMOUSE

11. GENE TARGETING

12. I VIII II X VI IX III V IV VII

13. Allele wild-type XI I II III IV V VI VII VIII IX X Allele null KO PGK-neo XI I II III IV V VIII IX X

14. TK Neo Targeting vector 1 2 3 Target locus

15. TK Neo 1 2 3 HOMOLOGOUS RECOMBINATION Neo 5’ probe 3’ probe Targeted locus

18. TK Neo 2 1 3 Targeting vector 1 2 3 Target locus

19. TK Neo 2 1 3 HOMOLOGOUS RECOMBINATION 1 2 3 Neo 2 1 3 Targeted locus

20. Neo 2 1 3 Espressione di Cre In cellule ES Screening per individuare cellule che hanno perso Neo ma mantengono gli esoni 1 e 2 e le regioni fiancheggianti loxP 2 1 3 Generazione di topi che presentano i siti loxP in entrambi gli alleli 2 1 3 2 1 3

23. active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. VENTILATION BRONCHODYNAMIC STUDIES active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. BEHAVIOR ACTIVITY MOTOR COORDINATION VENTILATION BRONCHODYNAMIC STUDIES VENTILATION BRONCHODYNAMIC STUDIES BEHAVIOR ACTIVITY MOTOR COORDINATION active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. BEHAVIOR ACTIVITY MOTOR COORDINATION VENTILATION BRONCHODYNAMIC STUDIES VENTILATION BRONCHODYNAMIC STUDIES BEHAVIOR ACTIVITY MOTOR COORDINATION BEHAVIOR ACTIVITY MOTOR COORDINATION BEHAVIOR ACTIVITY MOTOR COORDINATION active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. BEHAVIOR ACTIVITY MOTOR COORDINATION VENTILATION BRONCHODYNAMIC STUDIES active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. BEHAVIOR ACTIVITY MOTOR COORDINATION VENTILATION BRONCHODYNAMIC STUDIES active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. BEHAVIOR ACTIVITY MOTOR COORDINATION VENTILATION BRONCHODYNAMIC STUDIES VENTILATION BRONCHODYNAMIC STUDIES active analgesic compounds. Tail Flick, Hot Plate and Plantar Test, to ascertain peripherally mediated responses to thermal stimulation caused by drugs. The Analgesy-Meter (paw pressure test) to assess response caused by force exerted on the normal & inflamed paw of a rat. The Plethysmometer, the "standard" instrument, quite popular in the pharmaceutical industry, for accurate measurement of the rat paw swelling. Directly connectable to the PC. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. • Most popular behavioural tests in the pharmacological investigations: • Avoidance, via the Automatic Reflex Conditioner, a microcontrolled unit equipped with rat & mouse cage. • Passive Avoidance (both step-through & step-down schemes) provided with rat & mouse cages, to assess recent memory and its alterations brought about by drugs. • Hole Board (planche à trous) to confront the animals (mice) with a new environment and evaluate their reactions. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. A family of Ventilators of original design, Cat. 7025 for Rodents, Cat. 6025 for Cats and Rabbits, Cat. 5025 for Dogs and Mammal of similar size, enable the research worker to ventilate the experimental animals according to Starling’s method in the most critical experiments without any problem. The Ugo Basile Ventilators are rugged, quiet in operation, electromagnetically silent, i.e., they do not broadcast RF noise. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • Activity Cage (assessment of motor activity), for preliminary screening on the action of stimulating and sedative drugs, plus a number of studies on social interaction, feeding, environment. • Rota-Rod Treadmills for Rodents have proved to be of great value in research on drugs which are potentially active on motor coordination. We have constant speed and accelerating models; the latter operate according to the method by B..J. Johns and D.J. Roberts. • MISCELLANEOUS • A VARIETY OF INSTRUMENTS ENCOMPASS THE EVER INCREASING RANGE OF INVESTIGATIONS ON ISOLATED PREPARATIONS AND EXPERIMENTAL ANIMALS: • The ECT Unit (electroconvulsive treatment) is specially designed for neurochemical and neuropharmacological research. Reproducible results and accurate determination of EC Threshold. • The Lesion Making Device, designed for the production of a localized lesion in small animal preparations, where DC is preferred to RF output. • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart" • The Multi-function Printer is a microcontrolled device designed for the acquisition of counting or timing data from six independent channels. The data can be printed out in real time or routed to the PC. The Win-DAS software is required. FOR FUTHER DETAILS SEE MORE ABOUT IT… • The Isolated Perfused Heart System, as originated by Oscar Langendorff, allows the examination of cardiac inotropic, chronotropic and vascular effects without the complications of an intact animal model. The Radnoti system, marketed by our company encompasses constant pressure & constant flow models as well as "working heart"