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The structure was determined at 1.0 Å resolution by single-wavelength anormalous diffraction 。

Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function. Alexey Teplyakiv, Sadhana Pullalarevu, Galina Obmolova, Victoria Doseeva, Andrey Galkin, Osnat Herzberg, Miroslawa Daueter, Zbigniew Dauter, and Gary L Gilliland

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The structure was determined at 1.0 Å resolution by single-wavelength anormalous diffraction 。

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  1. Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function Alexey Teplyakiv, Sadhana Pullalarevu, Galina Obmolova, Victoria Doseeva, Andrey Galkin, Osnat Herzberg, Miroslawa Daueter, Zbigniew Dauter, and Gary L Gilliland Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute and the National Institute of Standards and Technology BMC Structural Biology 2004, 4:5 PDB ID ：1RW1

  2. 前言 • The yffB ( PA 3664 ) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kD molecular weight with a marginal sequence similarity to arsenate reductase from E. coli。 • The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein。

  3. 結果 • The structure was determined at 1.0 Å resolution by single-wavelength anormalous diffraction 。 • The fold is very similar to that arsenate reductase, which is an extension of the thioredoxin。

  4. 所探討的生物問題 • Yffb 蛋白和 arsenate reductase 在序列上的相似性，但最後結果發現在功能上被認為是 glutathione-dependent thiol reductase

  5. 到期末要做的事 • 試著了解 yffb protein 、arsenate reductase、glutathione-dependent thiol reductase 的差異處和相似處 • 是否也有相似的其他案例 • 從現在到期末所學任何方法

  6. 可能得到的知識 • 對蛋白質進行基本的了解和解析 • 知道從何處找到關於蛋白質結構相關之網站

  7. 我嘗試做的一些分析 • 在 ExPASy Molecular Biology Server進行序列比對 • 在 NCBI 進行序列比對 • 在 EBI 比較之間的 conservation • 最後利用 pdb 看他們的親源關傒

  8. >gi|32038782|ref|ZP_00137054.1| COG1393: Arsenate reductase and related proteins, glutaredoxin • family [Pseudomonas aeruginosa UCBPP-PA14] • Length = 115 • Score = 226 bits (577), Expect = 1e-58 • Identities = 109/114 (95%), Positives = 110/114 (96%) • Query: 1 TYVLYGIKACDTXKKARTWLDEHKVAYDFHDYKAVGIDREHLRRWCAEHGWQTVLNRAGT 60 • TYVLYGIKACDT KKARTWLDEHKVAYDFHDYKAVGIDREHL+RWCAEHGWQTVLNRAGT • Sbjct: 2 TYVLYGIKACDTMKKARTWLDEHKVAYDFHDYKAVGIDREHLQRWCAEHGWQTVLNRAGT 61 • Query: 61 TFRKLDEAQKADLDEAKAIELXLAQPSXIKRPVLELGGRTLVGFKPDAYAAALA 114 • TFRKLDEAQKADLDEAKAIEL LAQPS IKRPVLELG RTLVGFKPDAYAAALA • Sbjct: 62 TFRKLDEAQKADLDEAKAIELMLAQPSMIKRPVLELGSRTLVGFKPDAYAAALA 115

  9. >gi|56460562|ref|YP_155843.1| Glutaredoxin family protein [Idiomarina loihiensis L2TR] • gi|56179572|gb|AAV82294.1| Glutaredoxin family protein [Idiomarina loihiensis L2TR] • Length = 114 • Score = 79.0 bits (193), Expect = 3e-14 • Identities = 38/101 (37%), Positives = 61/101 (60%) • Query: 4 LYGIKACDTXKKARTWLDEHKVAYDFHDYKAVGIDREHLRRWCAEHGWQTVLNRAGTTFR 63 • +YGIK CDT KK+ WLD+++VAY+F D + + +E + W + +++LN+ T++R • Sbjct: 4 VYGIKNCDTVKKSLKWLDKNEVAYEFIDVRQQPLQKETVSGWLQKLPAESLLNKRSTSWR 63 • Query: 64 KLDEAQKADLDEAKAIELXLAQPSXIKRPVLELGGRTLVGF 104 • L + QK+ +D EL QP+ KRP++E G GF • Sbjct: 64 NLSDEQKSVVDANAIAELVAEQPTLFKRPLVESGQAVHCGF 104

  10. 此篇報告利用的分析 • X-ray diffration • Protein data bank • MALDI

  11. 經過分析得到的結果 • 至少知道蛋白質基本結構組成 • 知道有哪些蛋白質結構的網站 • 未來也許會有可能做蛋白質分析

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