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Arthritis Imaging Project

Arthritis Imaging Project. Project Goals. Quantified evaluation of arthritis in mice using Micro-CT imaging of excised naïve, mild and severe paw samples to identify: Joint Cortical Bone Volume (JCBV) Cortical Bone Density (CBD) Periosteal New Bone Volume (PNBV).

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Arthritis Imaging Project

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  1. Arthritis Imaging Project

  2. Project Goals Quantified evaluation of arthritis in mice using Micro-CT imaging of excised naïve, mild and severe paw samples to identify: • Joint Cortical Bone Volume (JCBV) • Cortical Bone Density (CBD) • Periosteal New Bone Volume (PNBV)

  3. Micro-CT Imaging ParametersGM-I X-O™ MicroCT • Cone beam x-ray source • X-ray source voltage = 75 keV • 50 µm pixel pitch with 3x magnification • 17 µm reconstructed voxel size • 1024 projections • 500 msec integration time • 390 µA total current • 0.15 Gy radiation dose • 8 minute acquisiton

  4. GM-I Study • Images • 3D Surface Rendering of Cortical Bone Naïve Severe

  5. GM-I Study • Images • Segmentation of MTP Joints 1 mm above and 2 mm below the joint

  6. GM-I Study • Images • Cross-Sections of Segmented Cortical Bone (A) and Periosteal New Bone (B) A Threshold: Cortical 2200 / New Bone 1500 B

  7. GM-I Study Results • Cortical Bone Density • Naïve 2622.67 • Mild 2727.67 • Severe 2659.981

  8. GM-I Study Results • Joint Cortical Bone Volume • Naïve • 0.36 mm3 (2nd) • 0.36 mm3 (3rd) • 0.36 mm3 (4th) S.D. = 0 • Mild • 0.48 mm3 (2nd) • 0.48 mm3 (3rd) • 0.50 mm3 (4th) S.D. = .0115 • Severe • 0.26 mm3 (2nd) • 0.22 mm3 (3rd) • 0.19 mm3 (4th) S.D. = .035

  9. GM-I Study Results • Periosteal New Bone Volume • Naïve • 7.35 mm3 (48%) • 15.30 mm3 (Total bone volume) • Mild • 7.12 mm3 (43%) • 16.26 mm3 (Total bone volume) • Severe • 7.67 mm3 (59%) • 12.9 mm3 (Total bone volume)

  10. GM-I Study Bone volumes and density were calculated using built-in software for quantification analysis

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