LAB SAFETY Section 1.3 c Biology Mrs. Baldessari

1. LAB SAFETYSection 1.3 cBiology Mrs. Baldessari

2. Objectives: • Identify and locate laboratory safety devices in the classroom. • Demonstrate proper/safe laboratory procedures. • Identify and know the function of various laboratory equipment. • Demonstrate proper laboratory techniques.

3. Introduction Safety in the laboratory should be first and foremost Accidents in a laboratory may have the potential of resulting in serious consequences. Common laboratory accidents include cuts, burns, and the inhalation of volatile fumes. The occurrence of each of these accidents, and others, can be minimized if simple safety precautions are followed.

4. When to wear goggles and aprons? • If using chemicals and flames

5. NO!!!!!!! YES!!!!!!!!! Personal Safety: Goggles

6. THINK! • Accidents in the lab can be prevented! • You are the most likely cause of an accident!

7. When you first enter the lab… • A. Go to lab and start experiments • B. try out your own experiments while you wait • C. read the lab and wait for the teacher’s instructions • D. go pick up chemicals and practice the lab • ANSWER: C

8. Read Lab First • Read the experimental procedure ahead of lab.

9. Disposing of Matches • The match should be quenched with water, NOT thrown into the waste crock directly. • Fires may occur.

10. No Horseplay

11. What should I wear in the lab… • if I am going to work with glassware? • Goggles

12. Closed Toed Shoes • The Safety Code of most schools will not approve of open foot ware; so avoid sandals or flip-flops

13. NO FLOPPIES • Avoid floppy garments; avoid things that dangle • Avoid long, loose hair styles for the same reason. • When Bunsen burners are in use, long hair sometimes catches on fire

14. Where’s the Safety Equipment? • Fire Extinguishers • Fire Blanket • Eye-wash Fountain • First-Aid Kit

15. Fire Safety • Small fires can be extinguished by covering them with a container, such as a beaker, to eliminate oxygen. • Moderately sized fires require the use of a fire extinguisher. • Large fires require the evacuation of the building and notifying the fire department. • If your clothing catches fire, the best extinguisher is water from the safety shower. • If a safety shower is not available, you can fall to the floor and cover yourself with a fire blanket. • Make sure you are aware of the location of the safety shower and fire blanket prior to performing any experiments. • Synthetic clothing fibers melt during combustion and produce hot liquids that stick to the skin. It is best to wear natural fibers, such as cotton, for fire safety when working in the laboratory.

16. the carbon dioxide extinguisher is red general purpose extinguishers. AVOID using a dry-chemical extinguisher on a fellow human being. Fire Extinguishers

17. Pull Pin Aim Fire Extinguisher

18. Fire Extinguisher • Squeeze the handle to begin operation.

19. Fire Blanket • Wrap it around the victim to smother the fire.

20. EYEWASH

21. EYE WASH • If you get something from the lab in your eye— • GET IT OUT IMMEDIATELY! • Run some water through the eyewash fountain before you use it. • Retract your eyelid (hold it open); • Don’t squint—this restricts water access. • Run fresh water over your eye for 20 minutes. • Go to the school nurse immediately afterward.

22. First Aid Kit • These items may be used temporarily for small injuries (say, a cut finger)Go to the school nurse immediately afterward. • Do not wait until the end of the day.

23. First Aid Kit • However, if blood is in evidence-- STAY AWAY! Let the victim apply his/her own bandage. • If blood is on the floor or lab bench, let trained personnel do the clean-up.

24. FUME HOOD • Use the HOOD for reactions that give off vapors, especially smelly vapors. • The draft of the HOOD will sweep away vapors so that the lab itself maintains reasonable air quality

25. Shower should be used for dire EMERGENCY only! If you (or a lab mate) is spilled chemicals, position yourself (or your lab mate) under the safety shower. Safety Shower

26. Pull the handle—a deluge of water will result. Safety Shower

27. The safety shower should also be used if you suffer a massive spill of a dangerous chemical on yourself, and need to get it off rapidly. Safety Shower

28. Broken Glass • Sweep it up right away • Don’t track in it all period.

29. Broken Glass • Place the broken glass in a crock.

30. If you need to leave the lab table to go to the bathroom, • A. Be sure someone is watching your lab station • B. shut off the gas valve and electrical equipment

31. Is it OK to… • A. eat in the lab • B. put your head in the fume hood • C. chew gum in the lab • D. drink in the lab • E. have horseplay in the lab • ANSWER: NO

32. Cut your finger Your clothing caught on fire You burned your finger Mercury ran out of a thermometer Don’t touch it Stop drop and roll Run it under cold water Clean the wound, then use a band-aid What is the first aid step to do if you…

33. All living organisms should be handled with… • RESPECT • That means do not throw things into their homes. • Do not tap on the aquaria. • Do not throw them on other students.

34. What should you bring back with you to the lab? • A. Book Bag • B. Something to write with • C. Lab sheet • D. Biology Book • E. Lunch • ANSWER: B and C

35. No UNAUTHORIZED experiments! These are terrible dangers in unskilled hands. NO!!!!!!!!!!!!!

36. No EATING or DRINKING in the LAB. A good practice is to assume everything in the lab is toxic. NO!!!!!!!!!!!!!!!

37. DO NOT drink from lab equipment. NOT EVEN distilled water. No!!!!!!!!!!!!!!!!!

38. When using pipets, DO NOT suck the liquid into the pipet directly by mouth. Pipets NO

39. DO use a rubber bulb to create a vacuum. Pipets YES

40. When to use the beaker tongs?

41. Should you use glassware that is… • Chipped • Dirty • Cracked • ANSWER? NO

42. Do NOT smell the flask directly. Testing of Odors

43. If you absolutely must test the odor, carefully waft the vapor from the flask toward your nose with your hand. Keep the flask quite distant from your face. Wafting YES

44. Remember NOT to hand the red-hot glass to another person, especially the teacher. HOT GLASSWARE

45. Make sure that reaction tubes, e.g. test tubes, are not directed toward yourself or other persons. The chemicals may splatter out the tube. Heating Test Tubes

46. Aim away from you and all people! Heating Test Tubes

47. DO NOT add WATER to CONCENTRATED ACID. The heat generated may cause splattering. Water to Acids NO

48. DO add ACID to WATER instead of the reverse order of addition. The heat generated will be less, but splattering still may occur. Do as you ought’a - add Acids to waoughta (water)

49. Working Safely in Biology • Remember you are responsible for your own safety as well as that of your teacher, classmates, and any live animals you handle.

50. Working Safely in Biology • Scientists working in a laboratory or in the field are trained to use safe procedures. • Laboratory work may involve contact with dangerous materials or organisms.