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Production of Invert Sugar using Lentikats B iotechnology

Production of Invert Sugar using Lentikats B iotechnology. Content. Our aim What is Lentikats Biocatalyst Production of Lentikats Biocatalyst Benefits of Lentikats Biotechnology Lentikats Biocatalyst in Food Industry Our guarantees Our partners Contacts.

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Production of Invert Sugar using Lentikats B iotechnology

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  1. Production of Invert Sugar using Lentikats Biotechnology

  2. Content Our aim What is Lentikats Biocatalyst Production of Lentikats Biocatalyst Benefits of Lentikats Biotechnology Lentikats Biocatalyst in Food Industry Our guarantees Our partners Contacts

  3. LentiKat’s a.s.in Food Industry • LentiKat’s a.s. follows up on nine year of research and development of Lentikats Biotechnology. • Our production know-how for commercial use of our Biocatalyst and for its industrial applications is patented internationally. • Our main focus is in intensification of production processes that leads to: • higher process yields • enhanced process stability, • Increased revenue, • Reduction of environmental footprint. Biotechnologies are changing the world…

  4. Lentikats Biotechnology Unique shape of the matrix and original immobilisation procedure enables to encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a hydrogel porous carrier from polyvinylalcohol (PVA). The advantages of PVA matrix are: excellent physical and mechanical characteristics that result in along-term mechanical stability hardly biodegradable no toxic effects Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from the bulk media and therefore can be used repeatedly. With biotechnologies to a better future…

  5. How does the Lentikats Biocatalyst work? A maximum surface in combination with the minimum volume of Lentikats Biocatalyst results in a substantial reduction of diffusion limitations associated with the transfer of substrate into the matrix and the product out of the matrix.

  6. Why polyvinylalcohol? It is an inexpensive material Encapsulation takes place under optimal, cell-sparing conditions It allows a production of hydrogel with excellent mechanical properties It does not have any side effects on fermentation It offers high capacity for the cell loading The process is stable and easy to control It is a non-toxic material Biotechnologies are changing the world…

  7. How is Lentikats Biocatalyst produced?

  8. Lentikats Biocatalysts • Biocatalyst Types for Use in Lentikats Encapsulation: • Free Cells • dead cells • viable, non growing cells • viable and growing cells • Free or Immobilised Enzymes. Lentikats Biotechnology allows to immobilise any type of enzyme • The size of Lentikats Biocatalyst pores varies depending on the size and diameter of the Biocatalyst due to: • PVA additive type (size of macromolecule) • additive concentration • biomass concentration

  9. Lentikats Biocatalysts Manufacturing Plant

  10. Laboratory Units for Encapsulation • For low-capacity production of encapsulates (especially for research purposes), a laboratory device is available

  11. What are the benefits? Matrix containing high concentration of biomass Higher yields (in comparison to conventional technologies using free biomass) Higher reaction rates Reduction of reaction tanks’ volume Operational costs savings (electricity, heat) Possibility of repeated usage Operational costs savings Enhanced resistance of modified and immobilised biomass Higher process stability Easy separation of biomass from reaction media Reduced costs for downstream processes (separation) Lower production of waste and by-products Environmentally friendly disposal

  12. ..and also… Shortening of the reaction times Much higher yieldsin comparison to conventional technologies(up to 5 – 15 % higher) Repetitive useof the Biocatalyst, easy separationfrom media (sieve separator) Higher productivityof the Biocatalyst Lower risk of contamination during the production process High stability and easy control of production process Biotechnologies are changing the world…

  13.       Technological set-up  - inlet of reaction media  - filling of Lentikats Biocatalyst  - medium with Lentikats Biocatalyst  - stirrer  - sieve separator  - outlet of separated media  - outlet of Lentikats Biocatalyst

  14. Lentikatsand food industry We currently offer solutions for the following processes: production of lactose free milk Lentikats Biocatalyst with β-galaktosidase activity production of invert sugar Lentikats Biocatalyst with invertase activity whey processing Lentikats Biocatalyst with β-galaktosidase activity for: production of D-galaktose from whey lactose (pharmaceutical quality) production of galacto-oligosaccharides from whey lactose production of special syrups Lentikats Biocatalyst with invertase activity for production of glucose-fructose syrups Lentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar and starch-based materials or glucose syrups from maltotriose or maltose syrup

  15. Lentikatsand food industry Following solutions are currently under development and testing: production of organic acids Lentikats Biocatalyst with immobilised bacteriaBacillus coagulansfor production of lactic acid from lactose, glucose and sucrose Lentikats Biocatalyst with immobilised filamentous fungiAspergillus nigerfor production of gluconic acid from sucrose and glucose Lentikats Biocatalyst with immobilised filamentous fungiAspergillus terreusfor production of itaconic acid from sucrose and glucose Biotechnologies are changing the world…

  16. Invert Sugar in Food Industry • Sucrose source – sugar beat, sugar cane • Mixture of glucose and fructose (sucrose hydrolysis) • Invert sugar as a product: • composition 33% sucrose, 35% glucose and 32% fructose »composition4% sucrose, 51% glucose and 45% fructose • invert sugar is more expensive than syrup »invert sugar is sweeter and contains also other monosaccharides apart from sucrose • large variety of applications: kids food, pastry, soups and sauces, candy and sweets, fruit drinks, dairy products.

  17. Invert sugar in Distilleries • Sucrose source – sugar beat, sugar cane • Mixture of glucose and fructose • molasses–benefits of sucrose hydrolysis • a longer shelf-life »possibility of a year-round operation for molasses distilleries • a lower water binding capacity – possibility to a achieve higher thickness prior to storage (80%) »lower demands for storage space and transportation

  18. Lentikats Biotechnology and production of invert sugar Lentikats Biocatalyst with invertase activity =immobilised saccharase (β-D-fructofuranosidase) saccharase source –Baker’s yeast (Candida utilis) invertase activity600-1000 g sucrose/kg LB/hr optimum temperature 30 - 35°C optimum pH 4.5 – 5.0 operational stability 600 – 1000 hodin 18

  19. Lentikats Biotechnology and production of invert sugar The active compound isimmobilised invertase for sucrose hydrolysisto glucose and fructose. - Standard activity assay: batch test standard sucrose solution with the concentration 100 g/l temperature 30°C pH 4.5 (10 mM acetate buffer) conversion time 50 min conversion rate 100%

  20. Case studies

  21. 1) Hydrolysis of sucrose solution Input parameters sucrose concentration450 g/l pH = 4.5 temperature = 30°C batch operation mode solution volume: 2 222 m3 Lentikats Biocatalyst activity1000 g SUC/kg LB/hr stability 900 hr amount1ton Invert sugar production Outlet • concentration: • sucrose10 g/l • glucose 220 g/l • fructose 220 g/l • volume2 222 m3 Invert sugar production • amount1 000 000 kg • 14 EUR/m3solution • 0.03EUR/kg invert sugar

  22. 2) Hydrolysis of sucrose solution Input parameters sucrose concentration450 g/l pH = 4.5 temperature = 30°C batch operation mode Solution volume: 2222 m3 Lentikats Biocatalyst activity 1000 g SUC/kgLB/hr stability 1000 hr amount 1 ton Ethanol production Output • concentration: • sucrose10 g/l • glucose 220 g/l • fructose 220 g/l • volume2 222 m3 Ethanol production • volume 615 333 litres a.a. • 14 EUR/m3solution • 0.05EUR/litre a.a.

  23. 3) Molasses distillery Input parameters sucrose concentration120 g/l pH = 4.5 temperature = 30°C continuous operation mode molasses volume: 8 333 m3 Lentikats Biocatalyst activity1000 g SUC/kg LB/hr stability 1000 hr amount 1 ton Ethanol production Output • concentration: • sucrose30 g/l • glucose 45 g/l • fructose 45 g/l • volume8 333 m3 Ethanol production • volume629 167litres a.a. • 4EUR/m3molasses • 0.05EUR/litre a.a.

  24. Summary – application of 1 ton of LB

  25. Investment and Investment Return Guarantees • You can easily verify our great results by testing our Biocatalyst in your or other facilitieswith the material of your choice. • Sample of Lentikats Biocatalyst free of charge • Our company provides assistance and full technical support. • Our company offers a long-term cooperation to companies, whose main concerns arereduced investment costs andmaximal process profitability. Biotechnologies change the world…

  26. We guarantee the following: • output parameters • operational stability of Lentikats Biocatalyst and its activity and productivity Investment and Investment Return Guarantees

  27. Our partners in the field of industrial applications

  28. Our partners in the field of research and development

  29. Thank you for attention Laboratory: INBIT LentiKat‘s a.s. Kamenice 34 625 00 Brno Czech Republic Tel.: + 420 511 205 288 Tel.: + 420 511 205 287 CompanyHeadquarters: Office Building ARGO LentiKat‘s a.s. Evropska 846/176a 160 00 Prague 6 Czech Republic Tel.: + 420 255 710 680 Fax: + 420 255 710 699 E-mail: info@lentikats.eu Production plant: LentiKat‘sa.s. Pod Vinicí 83 471 27 Stráž pod Ralskem Czech Republic Tel.: + 420 487 851 393 Fax: + 420 487 851 399 E-mail: info@lentikats.eu

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