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A comparison study between natural and synthetic scaffolds, and their interaction with cells

A comparison study between natural and synthetic scaffolds, and their interaction with cells. Elaine Steinke Mentor: Milind Gandhi Advisor: Dr. Frank K. Ko. Background – Definitions.

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A comparison study between natural and synthetic scaffolds, and their interaction with cells

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  1. A comparison study between natural and synthetic scaffolds, and their interaction with cells Elaine Steinke Mentor: Milind Gandhi Advisor: Dr. Frank K. Ko

  2. Background – Definitions • Tissue Engineering – the application of principles and methods of engineering and life sciences toward fundamental understanding of structure-function relationships in normal and pathological mammalian tissues and the development of biological substitutes to restore, maintain or improve tissue function (NSF, 1988) • Tissue Scaffolds – a mechanically stable structure which is capable of functioning biologically in the implant site (Ko et al, 2001)

  3. Two kinds of scaffolds

  4. Hypotheses • First hypothesis: It is possible to fabricate nanofibrous scaffolds from combinations of silk and collagen, and PLAGA and collagen. • Second hypothesis: Protein based scaffolds promote cell attachment and proliferation better than synthetic polymer scaffolds. (Results pending.)

  5. Scaffold Preparations • Materials • Procedure Electrospinning schematic, drawn by Jason Michael Lyons, 2004

  6. SEM observations Silk (7%) + collagen (1%) in HFIP (5000X) Silk (7%) dissolved in HFIP (5000X)

  7. SEM observations PLAGA (15%) + collagen (1%) in HFIP (5000X) PLAGA (15%) in HFIP (5000X)

  8. Statistics

  9. Conclusions, Part One • It is possible to fabricate nanofibrous scaffolds from combinations of silk and collagen, and PLAGA and collagen, using the solvent hexafluoroisoproponol. • Average fiber diameters were measured, and found to be of acceptable sizes to continue.

  10. Cell Preparation and Culture • The cell line being used is rat heart endothelial cell. • Approximately 350,000 cells are initially seeded on the scaffolds. • After 4 hours, scaffolds are transferred to new plates, where any seeded cells will continue growing. • Cell numbers are counted on each of the scaffolds and recorded as day zero.

  11. Future Work • Count cells on days three, seven and fourteen • Observe scaffold-cell interactions using the scanning electron microscope on days three, seven and fourteen

  12. Acknowledgements Dr. Sun’s lab members, Eda Yildirim and Saif Khalil

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