1 / 10

HIV GP120 Envelope Protein

HIV GP120 Envelope Protein. Objectives : Observing consensus sequences of different strains Structure analysis . GP120 Function. GP120 involved in infection of human T-cells. HIV TYPES. HIV Type I. SIV. HIV Type II. HIV Type III. Human viruses. Simian virus. GP120 Sequence analysis.

faunia
Download Presentation

HIV GP120 Envelope Protein

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. HIV GP120 Envelope Protein • Objectives : • Observing consensus sequences of different strains • Structure analysis

  2. GP120 Function GP120 involved in infection of human T-cells

  3. HIV TYPES HIV Type I SIV HIV Type II HIV Type III Human viruses Simian virus

  4. GP120 Sequence analysis Sequence analysis shows a high mutation rate with preserved large consensus regions not only between strains, but also between different types

  5. GP120 Sequence analysis HIV Type I shows additional sequence of 7 aa in it’s consensus sequence in a consensus region in acids 27-33 consensusLWVTVFYGVPVWKEATTTLFCATDNK-------NTWATHACVPTDPDYQEVELNNVTENF 53 HIV1LWVTVYYGVPVWHDADPVLFCASDAKahsteahNIWATQACVPTDPSPQEVFLPNVIESF 92

  6. Two structures of GP120-CD4 from HIV 1 were analyzed Hiv-1 Yu2 isolate Hiv-1 Hxbc2 isolate

  7. Difference between the proteins The similarity is shown, using protein blast Score = 566 bits (1460), Expect = e-161Identities = 276/321 (85%), Positives = 289/321 (89%), Gaps = 8/321 (2%)

  8. Structure of conserved region GP120 Hxbc2 isolate Preserved region marked in yellow CD4

  9. Docking of GP120 Clustered solution of Dimers With current approach GP120 exists as a trimers or tetramers on the membrane surface. According to J. Biol. Chem., Vol. 275, Issue 45, 35137-35145, November 10, 2000

  10. Conclusions • Preserved sequences found • GP120-CD4 theoretical docking performed, clustered solutions found • The results provide a basis for further research

More Related