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Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes

Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes. Mitchell Lerner, Brett Goldsmith, Joe Mitala , Bohdana Discher , Paul Rhodes, A.T. Charlie Johnson University of Pennsylvania APS March 2010. Biological Olfaction.

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Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes

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  1. Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith, Joe Mitala, BohdanaDischer, Paul Rhodes, A.T. Charlie Johnson University of Pennsylvania APS March 2010

  2. Biological Olfaction http://nobelprize.org/ Number of discernable smells is much greater than the number of types of olfactory receptor cells (350 sensor types detect >10,000 odors for humans)

  3. Experimental Objective http://nobelprize.org/ Integrate biology with nanotechnology

  4. Nanotube Transistors Use standard techniques to construct state of the art CNT transistors

  5. Nanotube Functionalization Olfactory receptor protein in micelles Bare Nanotube Diazonium salt EDC NHS NTA

  6. Experimental Design:Attachment 1mm Confirmation of protein attachment to nanotubes

  7. Reproducible Sensing blue arrow – inert gas flush green arrow – eugenol exposure curves from 3 experiments showing a reproducible response to eugenol for mOR174-9 mOR 174-9 responds to eugenol in vivo

  8. Sensor Timing • experiment showing a response to cyclohexanone for mOR 256-17 • mOR 256-17 responds to cyclohexanonein vivo • Initial response k-1 3s, secondary response is 30s • Recoveryk-1 is 30s.

  9. Concentration Dependence • experiments showin responses formOR 203-1 • mOR 203-1 responds to 2-heptanone in vivo • fMRI studies show identical responses to 2-heptanone and n-amyl acetate in mice

  10. Each of the three proteins gives a different set of reproducible, concentration dependent responses mOR 256-17 mOR 174-9 mOR 203-1

  11. Summary We reproduced many properties of biological sensing in an electronic device. Proteins are maintaining some features found in vivo First use of G-protein coupled receptors in electronic sensors Potential for application in hazardous material detection and pharmaceutical testing

  12. Acknowledgements DARPA RealNose Project

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