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Polymerase chain reaction AMPLIFYING DNA

Polymerase chain reaction AMPLIFYING DNA. What do you need to replicate DNA?. http://www.youtube.com/watch?v=uDumZT5z5R8. http://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.html. Which DNA SEQUENCE IS AMPLIFIED?. Tandem repeats (VNTR).

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Polymerase chain reaction AMPLIFYING DNA

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  1. Polymerase chain reactionAMPLIFYING DNA • What do you need to replicate DNA? http://www.youtube.com/watch?v=uDumZT5z5R8

  2. http://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.htmlhttp://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.html

  3. Which DNA SEQUENCE IS AMPLIFIED?

  4. Tandem repeats (VNTR)

  5. Tandem repeat genes 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’

  6. Determining your allelescreating dna fingerprint using gel electrophoresis

  7. Sample gel 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’ Ladder Suspect 1 Suspect 2 Crime Scene

  8. How unique are you?

  9. RflpRestriction fragment length polymorphism

  10. EXAMPLE USE TTCC RESTRICTION ENZYME DNA SAMPLE ONE: ACTGTTCCACACTTCCGCACATCTTAATTCC DNA SAMLE TWO: GTTCCATGCCGAGTACGTTCCATGCTA

  11. I am your father….well maybe

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