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Basic science as it applies to forensic analysis

Basic science as it applies to forensic analysis. Rebecca Hailey, Florida State College - Jacksonville C hemistry Yousef Hijji , Morgan State U Chemistry Rachel Mohr, WVU Forensic Science Martin Overly, WVU Forensic Science Elizabeth Pollock, Richard Stockton College of NJ, Chemistry

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Basic science as it applies to forensic analysis

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  1. Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry YousefHijji, Morgan State U Chemistry Rachel Mohr, WVU Forensic Science Martin Overly, WVU Forensic Science Elizabeth Pollock, Richard Stockton College of NJ, Chemistry Jacqueline Speir, WVU Forensic Science Shuo Wei, WVU Biology

  2. Target audience • Introductory biology • Second semester general chemistry • Second semester general, organic, biological chemistry • Sophomore level forensic science students

  3. Learning goals and outcomes Learning Goals Learning Outcomes Will be able to predict reactions between given enzymes and substrates Will be able to interpret enzyme assay test results Will be able to predict the consequences of the addition of an inhibitor Will be able to relate the theoretical understanding of enzymes to real life applications • Students will learn about enzyme binding and specificity • Will understand the role of inhibitors • Apply knowledge to novel situation

  4. What do we need to know to solve this case? What do we know about saliva? How is it different from other body fluids?

  5. Which of the following components of saliva should we target for detection? • Water • Salts • Carbohydrates • Food particles • Salivary enzymes = amylase

  6. Demonstration of Phadebas test for the presence of amylase

  7. Which of the following is a positive test for saliva?

  8. How enzymes workIdentify the components a-d enzyme substrate active site product

  9. How enzymes workIdentify the components a-d substrate active site product enzyme (e.g. amylase)

  10. How enzymes workIdentify the components a-d active site product Substrate (e.g. starch) enzyme (e.g. amylase)

  11. How enzymes workIdentify the components a-d product substrate (e.g. starch) active site enzyme (e.g. amylase)

  12. How enzymes workIdentify the components a-d product substrate (e.g. starch) active site enzyme (e.g. amylase) animation

  13. Which one(s) of the following contains molecules which can serve as substrates for amylase? • Butter • Dietary fiber/cellulose • Jolly Ranchers • Egg whites • Potato chips

  14. Has the suspect been cleared, or looking increasingly guilty?

  15. Pharmaceutical case study You are a scientist at a pharmaceutical company and you are tasked with coming up with a pill that will mimic the Atkins diet (but, still allowing dieters to consume carbohydrates).  What do you need to know to do your job?

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