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Background

Molecular differences resulting from larval growth conditions in Aedes aegypti David Price Disease Vector Molecular Biology Lab NMSU PI Immo Hansen. Background. Aedes aegypti is an important disease vector Relatively safe model for malaria Plasmodium gallinaceum Existing Reference Genome.

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Background

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  1. Molecular differences resulting from larval growth conditions in Aedes aegyptiDavid PriceDisease Vector Molecular Biology LabNMSUPI Immo Hansen

  2. Background • Aedes aegypti is an important disease vector • Relatively safe model for malaria • Plasmodium gallinaceum • Existing Reference Genome

  3. Aedes/Dengue Situation http://www.healthmap.org/dengue/index.php

  4. Small vs Large Mosquitoes • Larval conditions affect adult insect • Crowding, food availability -> Changes in adult size, time to reach adulthood, immunity, reproductive success, energy stores, life span, fecundity on first blood meal, vectorial capacity

  5. Fat Body

  6. Bloodfeeding • Purdue Medical Entomology PBM- Post Blood Meal NBF- Not Blood Fed

  7. Hypothesis • Significant metabolomic, transcriptional and metabolic differences exist in the fat body between small and large mosquitoes, before and after blood meal.

  8. Methods • Metabolic • CO2 production • Metabolomic • GC/MS, LC/MS, LC/MS/MS analysis • Transcriptomic • RNA-Seq approach using Illumina sequencing

  9. Metabolomics • Questions: • Map metabolic pathway up/down regulation after blood meal Price et al, unpublished data

  10. Metabolic • Questions: • Do small and large mosquitoes burn energy at the same rate? • Are the substrates being used for energy the same? • In conjunction with other work: • Is there a difference in how far energy stores go?

  11. Metabolic Price et al, unpublished data, Sable Systems

  12. Transcriptomic • Questions: • Determine changes in gene expression between mosquitoes raised under different nutrient conditions • What patterns of expression are present?

  13. Conclusions • Used metabolomic, metabolic, and transcriptional descriptive work to identify differences between small and large mosquitoes; pre and post bloodmeal. • Immune regulation is curious • Results of small vs large vectorial capacity experiments have been conflicting • Signs of autophagy • No apparent metabolic difference

  14. Hansen Lab/Collaborators • Alexander Ulanov – University of Chicago, Urbana • Wayne Van Voorhies - NMSU molecular Biology • NIAID/NIH

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