Nanbiosensor Working Group, Nov. 19 th , 2002

1. Nanbiosensor Working Group, Nov. 19th, 2002 Convener: Antje Baeumner Participants: +Evangelyn Alocilja +Larry Branen +Paul Dawson +Richard Durst +Joseph Irudayari +Zhiko Kounev +Greg McCarty +Jerzy Nowak +Daniel Schmold +Ruixiu Sui +U. Sunday Tim +John Vetelino +Guigen Zhang www.nseafs.cornell.edu

2. Background and Rational of Nanobiosensors • Bioanalytical Nanosensor • using biology or used for biology • Collection, interaction, sensor platform, response • - Biosensor system, portable and non-portable

3. Background and Rational of Nanobiosensors • Characteristics of Biosensors: • - single use: remote, small, portable, rapid response (real time) and processing, • specific, quantitative • Laboratory: more specific (i.e. subtyping capability) • non-portable, high throughput, quantitative • Multichannel • Sensitive • Reliable • Accurate • Reproducible • Robustness • Stability • 6 more slides on background and rational

4. Background and Rational of Nanobiosensors Pathogen Detection: Foods, Plants, Animals, Fruits & Vegetables Plant/Animal Production, Transgenics Cloning BioProcessing, Foods , Industrial Products (BioProcessing, Food Quality + Safety) BioSensor, (Biosecurity) Agrosecurity Environmental Processing (Environmental Monitoring) Sustainable Agriculture (Sustainable Rural Community) • 6 more slides on background and rational

5. Specific Objectives in Bioanalytical Nanosensors 1. Novel nanomaterials adaptable to agricultural & food systems (limited sample preparation, complex systems (dirty), more robust) 2. Novel recognition mechanisms based on the nanoscale (fundamental studies, non-biomaterials, biomimetics, carbon nanotubes, MIP, recombinant – genetically engineered) 3. Novel integration mechanisms of transducer and recognition element (SAM, Directed/guided assembly, new immobilization (chemical, biological and electrical) 4. Novel transduction mechanisms (mechanical, impedance, piezoelectric, optical, electrochemistry, transistor) 5. Integration of NEMS remote receive/transmit systems (embedded on the chip, satellite interaction, GPS, remote powering biopowering) Education about Nanotechnology as overall educational topic area for initiative

6. Potential Outcomes & Impacts in Nanobiosensors

7. Crosscutting Issues • Collection: Transport, Bioseparation, Microfluidics, Bioselective surface • Sampling – representative sample (statistically significant) • Recognition: Bioselective Surface, Microfluidics, Nanomaterials, Nucleic Acids • specificity (saturation,time, fouling) • Transduction: Bioselective Surface, Microfluidic, Nanomaterial • Integration to bioselective surface • Data Processing:Nanomaterials

8. Crosscutting Issues (continued) Drug delivery: Sensing if release is required, where to release NanoBioprocessing: Modeling: Models for new nanosensors nanosensors

9. Estimated Research Budget for Bioanalytical Nanosensors Fundamental Theme area Centers of Research Education Total research challenge excellence infrastructure [in million $] (applied) (equipment) 1 1* 1* 2 1* 1* 3 1* 1* 4 1* 1* 5 0.4** 0.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000 $8.8 1 1* 1* 4 regional 2 1* 1* centers 3 1* 1* (5 year) 4 1* 1* $10/year 5 0.4** 0.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000 $18.8 1 2 3 4 5 • 1* • 1* • 1* • 1* • 5 0.4** • * 3 projects per year at $300,000 • ** 2 projects per year at $200,000 $4.4 1 1* 1* 4 regional 1* 2 1* 1* centers 1* 3 1* 1* (5 year) 1* 4 1* 1* $10/year 1* 5 0.4** 0.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000 $22.8 1 1* 1* 4 regional 1* 0.2 2 1* 1* centers 1* 0.2 3 1* 1* (5 year) 1* 0.2 4 1* 1* $10/year 1* 0.2 5 0.4** 0.4** 0.2 * 3 projects per year at $300,000 ** 2 projects per year at $200,000 $23.8

10. Biosensors: Definition of Biosensor Systems: • Bioanalytical Nanosensor • using biology or used for biology • Collection, interaction, sensor platform, response • - Biosensor system, portable and non-portable • Characteristics of Biosensors: • remote, small, portable, rapid response (real time) and processing, single use, • Specific, quantitative • Laboratory base more specific (I.e. subtyping capability) non-portable, high throughput • quantitative • Multichannel • Sensitive • Reliable • Accurate • Reproducibility • Robustness • Stability

11. Rapid sensor (min or less) • Simple • In-situ (real-time) • Detection at farm-level, and throughout food processing and distribution chain, • including consumer • Non-invasive (non-destructive) • Remote • Telemetric • we include FOOD SAFETY • HACCP • Examples: • implanted sensors in farm animals • Monitoring of pathogens throughout the farm for early recognition of disease • Tracing sources of “contamination” (typically done in the lab) • Sample egg wash water, telemetric detection and automatic adjustment of disinfectant • Fish farms • Mycotoxin levels in grains • Toxins from pathogenic organism Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture

12. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture • - GMO detection throughout processing/distribution including consumer • Detection the function of the immune system in animals to detect disease resistance • (production efficiency vs. disease) • rapid screening of clones • “SMART” plants – plants sensor • Production process: • increase yield, productivity by monitoring the animals and plants • Root/soil interface investigation • (ethylene ripening) • IPM • Ask experts in this field • Specifics demanded of biosensors • GMO --- rapid, simple etc. • others fast, but in the lab is sufficient

13. BioProcessing, Food Quality + Safety • Monitoring of Food Quality + Safety • Contamination, physical properties • - heavy metals, pesticides • - oxidation • - metabilites, amonia etc. • - antioxidants • - texture • On-line monitoring of bioprocessing (proteins, cheese) • Monitoring imported foods • Characteristics: • - both, portable and lab Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture

14. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture • Take everything from Pathogen Detection

15. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture • Environmental Monitoring, (quality) • Water • Surface, • ground, • watershed • Waste water • Air • Odor • Spores, • particulates • allergens • Soil • Nutrient monitoring in soils (phosphorus, carbon, nitrogen) • Characteristics of biosensors needed • portable • especially remote monitoring, since geographically dispersed

16. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture • Sustainable Rural Community • Environmental Monitoring, (quality) • Production • BioProcessing • ALL OF them fit into this category • Having nanosensors helping in making decisions, not only for monitoring • Even opens up opportunities between rural + urban discussions • Agriculture in the classroom • Nanosensors as example of teaching tool, I.e. high tech to Agriculture, to K-12 • Decision making community, water, air, waste management