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Toll-like receptor 4 polymorphism influence on the development of opportunistic diseases

Institutes: Ukrainian Medical and Dental Academy, Regional HIV/AIDS Prevention and Control Center, Poltava Ukraine. Toll-like receptor 4 polymorphism influence on the development of opportunistic diseases in HIV-infected patients. The presenting author is Tetiana Kyrychenko.

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Toll-like receptor 4 polymorphism influence on the development of opportunistic diseases

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  1. Institutes:Ukrainian Medical and Dental Academy, Regional HIV/AIDS Prevention and Control Center, Poltava Ukraine Toll-like receptor 4 polymorphism influence on the development of opportunistic diseases in HIV-infected patients The presenting author is Tetiana Kyrychenko Authors: G. Dubynska, T.Koval, I.Kaidashev, V.Korshenko

  2. Ukraine has one of the fastestgrowing HIV/AIDS  epidemics in the world and the highest in allof Europe. Late 2011 Ukraine numbered 210,000 HIV-positivepersons. Dynamics of officially registered new cases of HIV-infectionin Ukraine (on 100 thousand population)

  3. Toll-like receptors (TLRs) have a key role in innate immunity and mutations in the genes encoding these receptors have been associated with increased or decreased susceptibility to infections.

  4. Objectives: • to study the prevalence of TLR4 Asp299Gly SNP among the healthy and HIV-positive persons in Ukraine; • to find out HIV clinical manifestations depending on TLR4 genotype variants. chromosome 9q32–33 Asp299Gly, A>G

  5. Methods Patients. The study included 180 antiretroviral treatment-naive HIV-1 infected patients. The stage of the disease was recorded in accordance with the expanded European AIDS case definition. Laboratory procedures. Routine CD-4 cell count was determined using conventional flow cytometry with fresh blood samples from all patients. Plasma HIV-RNA was also routinely measured by the polymerase chain reaction (PCR) (Real time HIV-1, Abbout). TLR4 genotyping was performed by real-time PCR using oligonucleotide primers(Tercik PCR system, DNA-technology, Moscow)

  6. Characteristic of a Cohort of 180 patients infected with HIV-1 and studied in Relation to SNP of TLR4

  7. Historyof opportunistic infections in 180 HIV-infected patients

  8. Historyof opportunistic infections in 180 HIV-infected patients in relation to SNP of the Toll-like receptor 4 gene (No. (%) of patients) *AG compared to AA, Mann-Whitney U-test

  9. Historyof opportunistic infections in 180 HIV-infected patients in relation to SNP of the Toll-like receptor 4 gene (No. (%) of patients) *AG compared to AA, Mann-Whitney U-test

  10. Distribution of the 299 alleles of the patients when they were grouped by CD-4 T-cell counts equal to or above and below350, 200 100 cells/mm³, respectively *Only patients with CD4 T-cell counts above 200cells/mm³ had the prevalence of the Asp299Gly polymorphism (12%) significantly higher than patients with CD4 T-cell counts below than 200 cells/mm³ (3.7%), p<0.05

  11. Detection of opportunistic infections in HIV-infected patients with different levels of CD4(No. (%) of patients) *AG compared to AA, X²-test, p<0.05

  12. Conclusions This study suggests a grater risk for development of active tuberculosis, bacterial pneumonia, herpes zoster, hairy leukoplakia, toxoplasmosis of brain, chronic hepatitis Cin patients with the Asp299Gly TLR4 polymorphism our results demonstrate the impact of a TLR4 genetic polymorphism on development of opportunistic infections for CD4 levels more than 100.

  13. Acknowledgments G.Dubynska, prof., department of infectious diseases, Ukrainian Medical and Dental Academy, Poltava, Ukraine T.Koval, prof., department of infectious diseases, Ukrainian Medical and Dental Academy, Poltava, Ukraine I. Kajdashev, prof., chair of research department, Ukrainian Medical and Dental Academy, Poltava, Ukraine V.Korshenko, chief doctor of Regional centre of HIV/AIDS Poltava, Ukraine

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