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Stool Cultures for Water Samples

Stool Cultures for Water Samples. Julie A. Ribes, M.D., Ph.D. UKHealthCare Clinical Microbiology Laboratory. What is Routine for Stool Cultures?. Salmonella spp . Shigella spp . Direct plating and by Selinite Broth enrichment Edwardsiella tarda. What is Routine for Stool Cultures?.

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Stool Cultures for Water Samples

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  1. Stool Cultures for Water Samples Julie A. Ribes, M.D., Ph.D. UKHealthCare Clinical Microbiology Laboratory

  2. What is Routine for Stool Cultures? Salmonella spp. Shigella spp. Direct plating and by Selinite Broth enrichment Edwardsiella tarda

  3. What is Routine for Stool Cultures? Campylobacter detection Campy plate with Microaerophilic incubation

  4. What is Routine for Stool Cultures? Aeromonas sp. Plesiomonas sp. Bacilluscereus All of these can be identified off of a BAP, MAC or an XLD Aeromonas B. cereus

  5. What is Routine for Stool Cultures? Toxigenic E. coli CDC now recommends that testing for E. coli 0157H7 and the other toxigenic E. coli species be performed on all patients being evaluated for diarrheal disease, not just patients with bloody diarrhea Sorbitol MAC and Toxin testing both should be performed

  6. Testing for Giardia and Cryptosporodium • Direct Fluorescence Antibody staining (Meridian Biosciences)

  7. Wolf Run Water Findings D13 BAP • Sweep oxidase to pick up • Aeromonas • Pleisiomonas • Spready colony worked up for possible Bacillus cereus

  8. Wolf Run Water Findings D13 MAC • Screen all clear colony types (non-lactose fermenters {NLF}) for possible Salmonella and Shigella • Usual stools, not too many NLF

  9. Wolf Run Water Findings D13 XLD • Usual coliforms are yellow • Need to screen black colonies for possible Salmonella spp. • Need to screen clear red colonies for possible Shigella spp.

  10. Wolf Run Water Findings D13 MAC Sorbitol • Pink colonies are sorbitol fermenters (usual flora) • Screen all clear colonies for Shiga toxin-producing E coli 0157H7

  11. Shiga toxin testing on Overnight Broth

  12. Wolf Run Water Findings D13 • CIN Agar • Usually not much growth • Need to screen “bulls eye” colonies with pink centers

  13. Potentially Significant Organisms Isolated • D13 8/24/10Bacillus cereus, Aeromonas sobria, Pleisiomonas shigelloides • D10 8/24/10Aeromonas sobria, Bacillus cereus • D14 8/24/10Bacillus cereus, Aeromonas sobria • D16 8/24/10Aeromonas caviae, Aeromonas sobria, Bacillus cereus • D18 8/24/10Bacillus cereus, Aeromonas hydrophila, Aeromonas schubertii, Aeromonas sobria, Aeromonas caviae

  14. Potentially Significant Organisms Isolated • D03 8/31/10Aeromonas hydrophila • D13 8/31/10Aeromonas sp., Aeromonas sobria. Bacillus cereus • D18 8/31/10Aeromonas sobria, Pleisiomonas shigelloides • D19 8/31/10Aeromonas sp., Aeromonas hydrophila • D25 8/31/10 Aeromonas sobria, Aeromonas caviae

  15. Wolf Run Water Findings D25 BAP • No spready colonies • Still had some Oxidase positive organisms to work up

  16. Wolf Run Water Findings D25 MAC • Pink and clear colonies • Still needed to look at the clear ones

  17. Wolf Run Water Findings D25 XLD • All yellow colonies, nothing to work up for Enterobacteraceae

  18. Wolf Run Water Findings D25 CNA • Smattering of Gram positives, nothing significant

  19. Aeromonas hydrophila Aeromonas caviae

  20. Citrobacter freundii

  21. Bacillus cereus

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