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Delve into the fascinating historical aspects of microbiology, tracing the evolution from Leeuwenhoek's discovery of living microbes to Pasteur's groundbreaking Germ Theory of Disease. Learn about key figures like Jenner, Lister, and Koch, and the Golden Age of Microbiology. Explore the significance of Koch's Postulates and other pivotal developments in the field, including the advent of vaccines, sterilization methods, and the electron microscope. Uncover the intricate journey of microbiology as a science, shaped by pioneers such as Ehrlich, Fleming, Watson, and Crick.
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Microbiology is the study of microorganisms which are not visible to the unaided eyes. • Hence require a Microscope • Examples: • Viruses • Bacteria • Fungi • Protozoa
ANTONY VAN LEEUWENHOEK • First to observe living microbes • Invented the compound microscope
SPONTANEOUS GENERATION Early belief that some forms of life could arise from “vital forces” present in non-living or decomposing matter, abio-genesis. organisms can arise form non-living matter, can be synthesized de novo
LOUIS PASTEUR (1822 - 1895) FATHER OF MICROBIOLOGY • French Chemistry Professor • Proposed GERM THEORY OF DISEASE
LOUIS PASTEUR(contd…) • Disproved spontaneous generation of microbes • Showed microbes caused fermentation • Also developed vaccine against anthrax, fowl cholera and rabies. • Pasteurizationtechnique • Developed methods of sterilization : steam sterilizer, hot air oven, autoclave • Liquid media for bacterial growth • Pasteur Institute , Paris
Edward Jenner • 1796 – First vaccine (smallpox)
Joseph Lister 1867 Antiseptic Surgery( Carbolic acid- Phenol)
Robert Koch (1843 – 1910) • Hediscovered the anthrax bacterium, tubercle bacilli & cholera bacilli.
Robert Koch (1843 - 1910) Koch’s Postulates – Criteria used to establish the link between a particular micro-organism and its disease
Koch’s Postulates • Causative agent must be present in all affected organisms • The agent must be capable of being isolated and cultured in pure form • When cultured agent is introduced to a laboratory animal, same disease must occur • The same causative agent must be reisolated from the affected animal • Antibodies directed against the organisms should be demonstrable in host
GOLDEN AGE OF MICROBIOLOGY1860 to 1900 During this period, rapid advances, headed by Louis Pasteur and Robert Koch, led to the establishment of microbiology as a science.
OTHER PIONEERS Paul Ehrlich- Chemotherapy Alexander Fleming – Penicillin Watson and Crick – DNA structure Ruska – Electron Microscope ……………..