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Propionic acid bacteria

Propionic acid bacteria. Olivia Rasigraf HMC 2012. General. Gram + , non- sporeforming Facultative anaerobes High GC Secondary fermenters Contain cytochromes Habitats: skin, cheese. Isolation. Lactate-yeast extract broth. Incubation at 30°C, dark. “ Emmentaler ” habitat. CO 2.

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Propionic acid bacteria

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  1. Propionic acid bacteria Olivia Rasigraf HMC 2012

  2. General • Gram+, non-sporeforming • Facultative anaerobes • High GC • Secondary fermenters • Contain cytochromes • Habitats: skin, cheese

  3. Isolation Lactate-yeast extract broth Incubation at 30°C, dark

  4. “Emmentaler” habitat CO2 Lactobacillus casei Streptococcus thermophilus Propionibacteriumshermanii 3 Lactate → 2 Propionate + 1 Acetate + 1 CO2 + 1 ATP

  5. Methylmalonyl-CoA pathway Lactate Pyruvate Acetyl-CoA Acetyl-P Acetate Oxidative pathway

  6. Methylmalonyl-CoA pathway Pyruvate Acetyl-CoA Acetyl-P Lactate Acetate Oxalacetate Propionate Malate Reductive pathway Fumarate Succinate Methylmalonyl-CoA mutase (B12)

  7. Acrylyl-CoA pathway Acetate Acetyl-P Pyruvate Lactate Propionate

  8. Results • All enrichments successful • Isolation of single colonies Brown colonies: Methylmalonyl-CoA pathway White colonies: Acrylyl pathway

  9. Results

  10. Questions?

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