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MLRS 242 Immunology Pat Reed Antibodies

MLRS 242 Immunology Pat Reed Antibodies. Serum proteins. General antibody structure. Enzyme digestion fragments. Protein structure of immunoglobulins. Early amino acid sequence experiments were unsuccessful—too much variation Multiple myeloma serum is 95% same antibody

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MLRS 242 Immunology Pat Reed Antibodies

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  1. MLRS 242ImmunologyPat ReedAntibodies

  2. Serum proteins

  3. General antibody structure

  4. Enzyme digestion fragments

  5. Protein structure of immunoglobulins • Early amino acid sequence experiments were unsuccessful—too much variation • Multiple myeloma serum is 95% same antibody • Bence-jones protein found in urine of myeloma patients is excess light chain • 110 amino acids highly variable, rest are quite constant • 5 different isotypes identified: based on type of heavy chain: G,D,E,M,A • Human light chains: 60% kappa (K) chains, 40% lambda (L) chains

  6. Ribbon model of antibody

  7. Detailed structure of antibody

  8. Ribbon model of variable region • Variable region contains highly variable connecting regions called complementarity-determining regions or CDRs • These regions are also shown to be the antigen binding sites

  9. Model of antibody

  10. Amino acid diversity of variable domains – complementarity- determining regions (CDRs)

  11. Antigen – antibody interaction

  12. Space filling model

  13. Hiv protease and Fab fragmentConformational change in Fab domain

  14. General structures of different antibody classes

  15. Subclasses of IgG

  16. Secretory IgA

  17. Receptor bound IgE

  18. Isotype – different species • Allotype – same species, different alleles • Idiotype – same species, different VH and VL domains

  19. Isotypic determinants – different species variation within the constant region

  20. Allotypic determinants – different constant regions within the same species – different alleles

  21. Idiotypic determinants – variations in the variable region within the same antibody type

  22. B Cell Receptor - BCR

  23. Fc receptors – bind Fc portion of antibody molecules

  24. Immunoglobulin superfamily of cell receptors – evolved from common ancestor gene

  25. More members of the Ig superfamily

  26. Monoclonal antibodies

  27. Many uses for monoclonal antibodies

  28. Antibodies can deliver drugs to specific targets

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