550 likes | 713 Views
Fig. 1-29. Inherited Disorders “Sporadic” Cancers Aging. Cystic Fibrosis Cholera. Cystic Fibrosis. Muscular Dystrophy. “Sporadic” Cancers Aging. Alzheimer’s Disease. Lysosomal Storage diseases. I-Cell Disease. Pg. 25. Drug Targets. Protein kinases. Membrane receptors. G proteins.
E N D
Inherited Disorders “Sporadic” Cancers Aging Cystic Fibrosis Cholera Cystic Fibrosis Muscular Dystrophy “Sporadic” Cancers Aging Alzheimer’s Disease Lysosomal Storage diseases I-Cell Disease Pg. 25
Drug Targets Protein kinases Membrane receptors G proteins Ion channels Protein “pumps” Bcl-2 Family proteins Telomerase Microtubules Transport vesicles Translation components tRNA synthetases Ribosomes Topoisomerases
Microscopy Light Microscopy Electron Microscopy
Light Microscopy Bright Field Phase-Contrast Differential Interference Contrast (DIC) Fluorescence
Electron Microscopy (EM) Scanning EM Transmission EM
5’ 3’ 5’ 3’
0.34 nm Major Groove Minor Groove Pg. 171
Topoisomerase Treated DNA
Topoisomerase Relieves Stress of Overwinding During DNA Replication
Topoisomerase is an Anti-Cancer Drug Target Camptothecin
Werner Syndrome 15 yrs. 48 yrs. Mutation in gene encoding RecQ helicase
Human Karyotype 247 M bp 47 M bp Pg. 179
RNA Molecules 5’ 5’ 3’ 3’
mRNA (gene expression) Transfer RNA (Translation) Ribosomal RNA (Translation) Small nuclear RNA (exon splicing) Micro RNA (Gene regulation) Small cytoplasmic RNA (protein transport) Ribozymes (numerous biochemical functions) Types of RNA Molecules
Protein Functions Enzymes Membrane transport Intercellular transport Hormones Receptors Nanomachines Antibodies Toxins Fibers Bioluminescence/Fluorescence
Amino Acid Side Chains (Pgs. 74-75)
alpha-helix ß-Sheet
Representations of Protein Tertiary Structure Wire Frame Backbone Space Filling Ribbon Pg. 132
Quaternary Structure Pg. 141
Protein Domains (Src protein)
Comparison of Protein Primary Structure Cyclin-dependent Kinase Proteins
Complexity of Human Gene Expression
RNA Synthesis OH OH OH Pg. 202 (Modified for RNA synthesis)
1 µm Pg. 233