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Double-Beam Spectrophotometer

Double-Beam Spectrophotometer. Introduction. Principle What is the Spectrophotometry? Purpose of the instrument. Types of Spectrophotometer. Device Description. Mirror. Light Source. Monochromator. Entrance and exit slit. Rotating Discs. Device Description.

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Double-Beam Spectrophotometer

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  1. Double-Beam Spectrophotometer

  2. Introduction • Principle • What is the Spectrophotometry? • Purpose of the instrument. • Types of Spectrophotometer.

  3. Device Description • Mirror. • Light Source. • Monochromator. • Entrance and exit slit. • Rotating Discs.

  4. Device Description • Sample cell & Reference cell in cuvettes. • Photo Detector (PMT). • Computer. • Optical Wedge.

  5. Applications • Determination of Alanine Aminotransferase (ALT/GPT) in clinical samples. • Determination of cholesterol in clinical samples. • Determination of glucose in clinical samples. • Determination of urea in clinical samples. • Determination of phosphate in clinical samples.

  6. End of the presentation THANKS FOR YOUR ATTENTION

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