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More Partial Digestion

More Partial Digestion. Answer Key BIO 330L Cell Biology Lab Dr. Lin. A)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb

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More Partial Digestion

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  1. More Partial Digestion Answer Key BIO 330L Cell Biology Lab Dr. Lin

  2. A)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 5, 6, 7, 8, 10, 11, 12, 13, 14, 18, 19 kb B)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 5, 6, 10, 11, 12, 13, 14, 16, 17, 18, 19 kb C)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 5,  6, 7, 10, 11, 12, 13, 14, 17, 18, 19 kb D)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 5,  6, 7, 10, 11, 12, 13, 16, 17, 18, 19 kb E)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 5, 6, 7, 8, 10, 11, 12, 16, 17, 18, 19 kb

  3. (A) B B B B B 5 1 1 10 1 1 (B) B B B B B 5 1 10 1 1 1 (C) B B B B B 5 1 1 10 1 1 (D) B B B B B 1 5 10 1 1 1 (E) B B B B B 1 5 1 10 1 1

  4. F)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 5, 6, 7, 8, 10, 11, 12, 13, 17, 18, 19 kb G)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 5, 6, 7, 10, 11, 12, 15, 16, 17, 18, 19 kb H)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 5,  6, 7, 8, 10, 11, 15, 16, 17, 18, 19 kb I)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 4, 5,  6, 7, 8, 9, 10, 15, 16, 17, 18, 19 kb

  5. (F) B B B B B 1 5 1 10 1 1 (G) B B B B B 1 1 5 10 1 1 (H) B B B B B 1 1 1 5 10 1 (I) B B B B B 1 1 1 1 5 10

  6. J)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 16, 17, 18, 19 kb K)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 5, 6, 7, 8, 9, 10, 11, 12, 17, 18, 19 kb L)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 5,  6, 7, 8, 9, 10, 11, 12, 13, 18, 19 kb M)  Construct Restriction Map(s) base on the following Partial Digestion data using BamH1: Uncut: 19 kb BamH1 (Complete digestion) = 1, 5, 10 kb BamH1 (Partial digestion) =1, 2, 3, 4, 5, 6, 7, 8. 9, 10, 11, 12, 13, 14, 19 kb

  7. (J) B B B B B 1 1 1 5 1 10 (K) B B B B B 1 1 5 1 1 10 (L) B B B B B 5 1 1 1 1 10 (M) B B B B B 5 1 1 1 1 10

  8. Partial Digestion Answer Key (Enrichment activity #7) BIO 330L Cell Biology Lab

  9. Construct Restriction Map(s) base on the following Partial Digestion data using EcoR1: • Uncut: 21 kb; • EcoR1 (Complete digestion) = 2, 3, 4, 5, 7 kb; • EcoR1 (Partial digestion) = 2, 3, 4, 5, 7, 8, 9, 10, 12, 14, 17, 21 kb • The following are some of the possible Maps that fit the Gel data: • 1) 4E5E3E2E7 = 7E2E3E5E4 • 2) 4E3E5E2E7 = 7E2E5E3E4

  10. (A) E E E E 5 2 7 4 3 (A) E E E E 3 5 2 7 4 Uncut: 21 kb; EcoR1 (Complete digestion) = 2, 3, 4, 5, 7 kb; EcoR1 (Partial digestion) = 2, 3, 4, 5, 7, 8, 9, 10, 12, 14, 17, 21 kb (5 not at end: no16; 3 not at end: no18; 2 not at end: no 19)

  11. Construct Restriction Map(s) base on the following Partial Digestion data • using EcoR1: • Uncut: 21 kb; • EcoR1 (Complete digestion) = 2, 3, 4, 5, 7 kb; • EcoR1 (Partial digestion) = 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 21 kb The following are some of the possible Maps that fit the Gel data [Both of them are circular]: Map 1: 4E5E3E2E7E = 5E3E2E7E4E = 3E2E7E4E5E = 2E7E4E5E3E = 7E4E5E3E2E = 7E2E3E5E4E = 2E3E5E4E7E = 3E5E4E7E2E = 5E4E7E2E3E = 4E7E2E3E5E Map 2: 4E3E5E2E7E = 3E5E2E7E4E = 5E2E7E4E3E = 2E7E4E3E5E = 7E4E3E5E2E = 7E2E5E3E4E = 2E5E3E4E7E = 5E3E4E7E2E = 3E4E7E2E5E = 4E7E2E5E3E

  12. 4 E E 4 E E E 3 7 5 5 E E 7 3 7 3 E 5 E 2 E E 2 4 Accept: Both maps above fit gel data E E 2 E E Reject: Cannot get 10, 4 & 2 cannot be together (no 6 in partial digest) Uncut: 21 kb; EcoR1 (Complete digestion) = 2, 3, 4, 5, 7 kb; (every fragments can be at the end =>circular; [21-7=14, 21-5=16, 21-4=17, 21-3=18, 21-2=19]) EcoR1 (Partial digestion) = 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, [14, 16, 17, 18, 19,] 21 kb (4,7,2 need to be together to get 13; 4,2 cannot be together, no 6)

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