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Microscope

Microscope. Basics. Always carry a microscope with one hand holding the arm and one hand under the base. Diaphragm. Ocular lens (Eyepiece). Nosepiece. Arm. Objectives. Stage. Stage Clips. Coarse Adjustment Knob. Fine Adjustment Knob. Light. Base. What’s My Power?

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Microscope

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  1. Microscope Basics

  2. Always carry a microscope with one hand holding the arm and one hand under the base. Diaphragm Ocular lens(Eyepiece) Nosepiece Arm Objectives Stage Stage Clips Coarse Adjustment Knob Fine Adjustment Knob Light Base

  3. What’s My Power? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective. Ocular lens (10X) Objectives (4X, 10X, 40X)

  4. WHAT’S MY POWER? • Low Power • Ocular lens = 10X • Objective = 4X • TOTAL magnification for LOW power = ______________ 40 X

  5. WHAT’S MY POWER? • Medium Power • Ocular lens = 10X • Objective = 10X • TOTAL magnification for MEDIUM power = ______________ 100 X

  6. WHAT’S MY POWER? • High Power • Ocular lens = 10X • Objective = 40X • TOTAL magnification for HIGH power = ______________ 400 X

  7. We can see better details with HIGHERthe powers of magnification, but we can’t see as much of the image. Comparing Powers of Magnification:

  8. Which of these images would be viewed at a higher power of magnification?

  9. Microscope pictures

  10. Compound Light Microscopes: • You will be using compound light microscope in several labs. • These microscopes have a maximum magnification of 400X • So you cannot see most of the organelles like ribosomes, golgi bodies, lysomes, etc. • More powerful microscopes are needed (2,000 X plus)

  11. Common Problem……AIR BUBBLES AIR BUBBLES AIR BUBBLES

  12. Stained Onion Cells • Can you identify the cell walls? • Can you identify any other organelles?

  13. Stained Cheek Cells Cell Membrane Nucleus Cytoplasm

  14. Elodea - Typical plant cells as seen with the light microscope

  15. Animal Cell

  16. Nucleus

  17. Rough ER

  18. Golgi Body

  19. Mitochondria

  20. Chloroplasts

  21. Ribosomes

  22. Cytoskeleton

  23. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly 5 – Place the slide on the stage and view it first with the LOW power objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. You do not need to use the stage clips when viewing wet-mount slides! How To Make A Wet-mount Slide … 1 – Get a clean slide and cover slip from your teacher. 2 – Place ONE drop of water/iodine in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water/iodine drop.

  24. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it! Let’s give it a try ... 1 – Turn on the microscope and then rotate the nosepiece to click the LOW power objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 4 – When you are done,turn off the microscope and put up the slides you used.

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