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Microscope

Microscope. Biology 101 Mrs. Miller. Types of Microscopes. Light microscope Compound lenses to magnify objects. Lenses refract light to make object appear closer. 40x, 100x, 400x Stereoscope Allows binocular viewing of larger specimens. Types of Microscopes. Scanning Electron Microscope

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Microscope

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  1. Microscope Biology 101 Mrs. Miller

  2. Types of Microscopes • Light microscope • Compound lenses to magnify objects. • Lenses refract light to make object appear closer. • 40x, 100x, 400x • Stereoscope • Allows binocular viewing of larger specimens.

  3. Types of Microscopes • Scanning Electron Microscope • Use electrons to magnify objects instead of light waves. • Can magnify up to two million times. • Transmission Electron Microscope • Uses electrons as well. • Electrons are passed through very thin specimens.

  4. Lenses • Three magnification lenses • Scanning • 4x magnification • Low power • 10x magnification • High power • 40x magnification

  5. Lenses • Ocular lens • In addition to the magnification lenses. • 10x magnification

  6. Magnification • To find the total magnification multiple the magnification of the lens by the magnification of the ocular lens.

  7. Focusing • Always start with the scanning objective. • Coarse knob to focus. • Once you’ve focused on scanning, switch to low power. • Coarse knob to refocus. • Switch to high power. • Fine knob to refocus.

  8. Focusing • Can’t see the specimen? • Is it too light or too dark? • Adjust the diaphragm. • See a line? • It is a pointer • Useful for pointing things out to partner or teacher. • Try twisting the eyepiece

  9. Drawing Specimens • Use pencil. • Drawing should be large enough to see detail. • Label items in drawing. • Write what magnification you are viewing the object at. • Label the object.

  10. Drawing Specimens Ameba (400x) Cell Membrane Nucleus

  11. Clean Up & Storage • Make sure the scanning lens is in place. • Wrap cords around the base. • Cover microscope. • Wash and dry slides before placing them back in slide box. • Cover slips are to be thrown away.

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