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Bee Venom Introduction: BIOMEDICAL APPLICATIONS AND RESEARCH

Bee Venom Introduction: BIOMEDICAL APPLICATIONS AND RESEARCH. Bee Anatomy. Fig 2. Anatomy of abdomen 1. Fig 1. Anatomy of Apis mellifera. [1] Winston, L. M. The Biology of the Honey Bee (First Harvard Press, 1987). Insect Sting. = Injection of up to 100  g protein

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Bee Venom Introduction: BIOMEDICAL APPLICATIONS AND RESEARCH

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  1. Bee Venom Introduction:BIOMEDICAL APPLICATIONS AND RESEARCH

  2. Bee Anatomy Fig 2. Anatomy of abdomen1 Fig 1. Anatomy of Apis mellifera [1] Winston, L. M. The Biology of the Honey Bee (First Harvard Press, 1987).

  3. Insect Sting = Injection of up to 100 g protein (or 0.10 mg or 0.0001 gram)

  4. Bee Venom: • Applications in the field of medicine • Basic protein research using venom

  5. Venom manufacturing for bee sting allergies is now funding research on other medical applications for venom products. NEW DIRECTIONS:

  6. Folk remedies and anecdotal reports suggest that honey bee venom has beneficial effects for patients with: Biomedical Applications: Multiple SclerosisArthritisInfectious Diseases

  7. 1. Does Honey Bee Venom (HBV) have useful antimicrobial activity? 2. Can the activity be separated from its toxic components? RESEARCH FOCUS:

  8. Synergy with Antibiotics Normal Bacterial Growth HBV only Penicillin only Optical Density With Penicillin and HBV Time

  9. Honey Bee Venom Composition • Hyaluronidase • Phospholipase • Melittin • Low Molecular Weight Compounds

  10. Honey Bee Venom Sac Dissection

  11. Venom Isolation • 1. Sac dissection • 2. Tissue grinding • 3. Centrifugation to remove tissue • 4. Filter to remove smaller particles • 5. Lyophilization • 6. Protein separations: • Chromatography • Electrophoresis

  12. Chromatography: Honeybee Venom Sample: 20mg/mL Melittin Phospholipase ??????? Hyaluronidase

  13. Electrophoresis Honey Bee Venom contains relatively few proteins Yellow Jacket and Hornet venoms aremore complex

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