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Issues in Cytokine Measures: Talking points

Issues in Cytokine Measures: Talking points. Debra Lyon RN FNP. Absolute or Relative. Multiplex More measures Approximation of a network? Substantial cost and sample savings ELISA

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Issues in Cytokine Measures: Talking points

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  1. Issues in Cytokine Measures: Talking points Debra Lyon RN FNP

  2. Absolute or Relative Multiplex More measures Approximation of a network? Substantial cost and sample savings ELISA Dynamic range (range over which there is a linear relationship between the cytokine concentration and the absorbance reading) is narrow.

  3. ELISA Gold-standard? More comparability Tried and true measure

  4. Multiplex • Flow cytometric multiplex arrays, also known as bead-based multiplex assays, • Employ proprietary bead sets which are distinguishable under flow cytometry. Each bead set is coated with a specific capture antibody, and fluorescence or streptavidin-labeled detection antibodies bind to the specific cytokine-capture antibody complex on the bead set.

  5. Sample Issues • Venipuncture • Plasma or serum? • Tubes? • Storage

  6. Problems for All • Low levels of circulating cytokines • Variability of cytokines over time • Can you compare across studies

  7. How to proceed? Conceptualization of multiplex and ELISA as “complementary tests, with the former being used more for discovery, while the latter may play a confirmatory role.” Xin Zhou, Maren S. Fragala, Janet E. McElhaney, George A. Kuchel. (2010). Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research. CurrOpinClinNutrMetab Care. Author manuscript; available in PMC 2010 October 15.Published in final edited form as: CurrOpinClinNutrMetab Care. 2010 September; 13(5): 541–547. doi: 10.1097/MCO.0b013e32833cf3bcPMCID: PMC2955626

  8. Bottom Line • Relationships among cytokines and sickness behaviors, symptoms and other phenomena of interest have resulted in better reproducibility in animal models than in human subjects.

  9. References Sean X. Leng, Janet E. McElhaney, Jeremy D. Walston, DongxuXie, Neal S. Fedarko, George A. Kuchel. (2008). ELISA AND MULTIPLEX TECHNOLOGIES FOR CYTOKINE MEASUREMENT IN INFLAMMATION AND AGING RESEARCH J Gerontol A BiolSci Med Sci. Author manuscript; available in PMC 2008 October 7.Published in final edited form as: J Gerontol A BiolSci Med Sci. 2008 August; 63(8): 879–884. PMCID: PMC2562869

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