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PstI

HindIII. EcoRI. AflIII. AvaI. PvuII. DraI. A. PstI. SpeI. stop. DraI. NcoI. EDEN. 5’. 3’. *. -46. +146. +535. +888. +1041. +1453. +1967. B. C. HindIII - EcoRI. HindIII - EcoRI. HindIII - PvuII. PstI - HindIII. PstI - HindIII. PstI - EcoRI. PstI - EcoRI. PstI - NcoI.

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PstI

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  1. HindIII EcoRI AflIII AvaI PvuII DraI A PstI SpeI stop DraI NcoI EDEN 5’ 3’ * -46 +146 +535 +888 +1041 +1453 +1967 B C HindIII - EcoRI HindIII - EcoRI HindIII - PvuII PstI - HindIII PstI - HindIII PstI - EcoRI PstI - EcoRI PstI - NcoI Eg1 Eg1 kDa kDa HindIII - PvuII HindIII - AvaI HindIII - AflIII p90 HindIII - DraI 97.4 PstI - SpeI PstI - Dra I 69 97.4 p90 53 EDEN-BP 69 46 1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 CTP GTP

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