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A. B. Nef peptides. TBE peptides. *. *. *. 250. 175. 150. *. *. 200. 125. 150. 100. IL-2 SFC/10 6 splenocytes. IFN- g SFC/10 6 splenocytes. 75. 100. 50. 50. 25. A. B. C. D. E. F. A. B. C. D. E. F. M nef. M nef. PBS. PBS. D nef. D nef. M nef. M nef. PBS.
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A B Nef peptides TBE peptides * * * 250 175 150 * * 200 125 150 100 IL-2 SFC/106 splenocytes IFN-g SFC/106 splenocytes 75 100 50 50 25 A B C D E F A B C D E F Mnef Mnef PBS PBS Dnef Dnef Mnef Mnef PBS PBS Dnef Dnef Mnef Mwt Mnef PBS Mnef Dnef C 400 Pooled Splenocytes 300 Pooled splenocytes, CD8+ cells depleted 250 200 IFN-g SFC/106 cells 150 100 50 A B C D E F