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Explore the effects of altitude and landscape structure on population genetics, covering factors like size, isolation, age, mating system, and selection. Questions about genetic differences, metapopulation structures, and clonal reproduction are addressed, with methods including AFLP analyses and GIS techniques.
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Population genetics in different contexts: Impacts of altitude & landscape structure Thomas Hahn Thomas.hahn@env.ethz.ch BioChange
Population genetic factors • Size • Isolation • Age • Mating system • Selection 1/8
Questions I (altitude) Are upper peripheral populations compared with central ones : 0) more isolated & smaller? 1) genetically depauperate? 2) genetically more differentiated? ? ? 2/8
? Questions I (pollination) • Population genetic differences among insect- and wind-pollinated species? 3/8
Methods I n = 30 1800 m 1200 m 10 x AFLP-Analyses 4/8
Questions II (altitude & landscape) • How are metapopulations structured along an altitudinal gradient? • What are directions, corridors & barriers for gene flow in metapopulations? From Holderegger & Wagner, 2008 (changed) 5/8
Methods II ¦ 1000 m ¦ 3 x 15 Subpopulations à 30 Individuals GIS 6/8
A further question • Differences in clonal reproduction among central and upper peripheral populations in B. media? 7/8
From Murray, 1976 Challenges • Two cytotypes in B. media in Switzerland! • Distribution of cytotypes within & among populations, regions or heights? • Gene flow among cytotypes? Flow cytometry analyses: 8/8