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Albumin Analysis by Potentiometric Method. Properties of Albumin. Half-life: 17 to 23 days Molecular weight: 68,000 Isoelectric point: 4.8. The Advantages of Potentiometric Method. 1. Integrate easily 2. Microfabricate easily 3. Real time response. Materials. Monomer
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Properties of Albumin • Half-life: 17 to 23 days • Molecular weight: 68,000 • Isoelectric point: 4.8
The Advantages of PotentiometricMethod • 1. Integrate easily • 2. Microfabricate easily • 3. Real time response
Materials • Monomer Dimethylaminoethyl methacrylate-methyl chloride:DMAEMMC Dimethylaminopropyl methacrylamide:DMAPMA • Crosslinking agent Tetraethylene glycol dimethacrylate:TEGDMA
Materials • Initiators Cumene hydroperoxide:CHP Tetramethyl ethylenediamine:TMEDA • Modification agent 2-mercapto ethanol 3-mercapto propionic acid n-dodecyl thiol
Materials • Electrodes: Working : Au polymer film Counter: Pt Reference: Ag/AgCl • Electrolyte: 0.5M KCl